Anticorps Polyclonal de lapin anti-MARK1

• Phare
• Validé par KD/KO

MARK1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(11)

Avis (1)

N° de cat : 21552-1-AP

Synonymes

Par1c, PAR1 homolog c, MARK, MAP/microtubule affinity-regulating kinase 1, MAP/microtubule affinity regulating kinase 1

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Galerie de données de validation

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  WB analysis using 21552-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21552-1-AP (MARK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21552-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21552-1-AP (MARK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21552-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21552-1-AP (MARK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21552-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21552-1-AP (MARK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 21552-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21552-1-AP (MARK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 21552-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21552-1-AP (MARK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21552-1-AP

  IP result of anti-MARK1 (IP:21552-1-AP, 4ug; Detection:21552-1-AP 1:1000) with mouse brain tissue lysate 3440ug.

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  IHC staining of rat testis using 21552-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 21552-1-AP (MARK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 21552-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 21552-1-AP (MARK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, cellules SH-SY5Y, tissu cérébral de rat, tissu rénal de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu testiculaire de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

21552-1-AP cible MARK1 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MARK1 Protéine recombinante Ag16225
• Nom complet: MAP/microtubule affinity-regulating kinase 1
• Masse moléculaire calculée: 795 aa, 89 kDa
• Poids moléculaire observé: 85-89 kDa, 72 kDa
• Numéro d’acquisition GenBank: BC113869
• Symbole du gène: MARK1
• Identification du gène (NCBI): 4139
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MARK1, also named as MAP-microtubule affinity regulating kinase 1, which form a subfamily of the calcium/calmodulin-dependent protein kinase (CAMP) group of kinases(PMID: 22670221). It is involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. MARK1 also act as a regulator of the neuronal migration and Wnt signaling pathway. It has 3 isoforms which molecular weight is 89,84,72 kDa. This antibody may detect all of the isoforms.

Publications

Species	Application	Title
human	WB	Mol Cell; An AMPK-Independent Signaling Pathway Downstream of the LKB1 Tumor Suppressor Controls Snail1 and Metastatic Potential.; Authors - Jonathan M Goodwin ; KD Validated
human	IP	Nat Commun; TNK1 is a ubiquitin-binding and 14-3-3-regulated kinase that can be targeted to block tumor growth.; Authors - Tsz-Yin Chan ; KD Validated
mouse	WB	Cell Rep; DIXDC1 Phosphorylation and Control of Dendritic Morphology Are Impaired by Rare Genetic Variants.; Authors - Vickie Kwan
Mouse	WB,IHC	J Neurosci; Unbiased proteomics of early Lewy body formation model implicates active microtubule affinity-regulating kinases (MARKs) in synucleinopathies.; Authors - Michael X Henderson
human	WB	Cell Commun Signal; Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression.; Authors - Abiola Abdulrahman Ayanlaja
rat	WB,IP	J Biol Chem; Postsynaptic density 95 (PSD-95) serine 561 phosphorylation regulates a conformational switch and bidirectional dendritic spine structural plasticity.; Authors - Qian Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Manish (Verified Customer) (06-09-2020)

The antibody works really well. We dilute it 10 fold and still get strong bands on western blots.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

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