• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-MARK2

MARK2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(16)

N° de cat : 15492-1-AP

Synonymes

ELKL motif kinase 1, EMK 1, EMK1, MARK2, PAR 1, PAR1 homolog, Par1b



Applications testées

Résultats positifs en WBtissu cérébral de souris, tissu cérébral de rat
Résultats positifs en IPtissu cérébral de rat
Résultats positifs en IHCtissu de cancer de la prostate humain, tissu cérébral humain, tissu testiculaire de souris
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

15492-1-AP cible MARK2 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène MARK2 Protéine recombinante Ag7884
Nom complet MAP/microtubule affinity-regulating kinase 2
Masse moléculaire calculée 88 kDa
Poids moléculaire observé 77-90 kDa
Numéro d’acquisition GenBankBC008771
Symbole du gène MARK2
Identification du gène (NCBI) 2011
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MARK2 is also named as EMK1(ELKL motif kinase 1), Par1b and belongs to the CAMK Ser/Thr protein kinase family. Par1b/MARK2 plays a critical role in neuronal polarity in the process of axon specification from multiple candidate neurites using primary cultures of mammalian hippocampal neurons(PMID:20194617). It has 16 isoforms produced by alternative promoter usage and alternative splicing with the molecular mass of 77-90kDa and can exsit as a dimer(PMID:16803889).

Protocole

Product Specific Protocols
WB protocol for MARK2 antibody 15492-1-APDownload protocol
IHC protocol for MARK2 antibody 15492-1-APDownload protocol
IP protocol for MARK2 antibody 15492-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

An AMPK-Independent Signaling Pathway Downstream of the LKB1 Tumor Suppressor Controls Snail1 and Metastatic Potential.

Authors - Jonathan M Goodwin
humanIP

Nat Commun

TNK1 is a ubiquitin-binding and 14-3-3-regulated kinase that can be targeted to block tumor growth.

Authors - Tsz-Yin Chan
  • KD Validated
MouseWB,IHC

J Neurosci

Unbiased proteomics of early Lewy body formation model implicates active microtubule affinity-regulating kinases (MARKs) in synucleinopathies.

Authors - Michael X Henderson

FASEB J

Cdc42 is involved in NC1 peptide-regulated BTB dynamics through actin and microtubule cytoskeletal reorganization.

Authors - Wenhui Su
ratWB

FASEB J

Regulation of the blood-testis barrier by a local axis in the testis: role of laminin α2 in the basement membrane.

Authors - Ying Gao
humanWB

J Cell Sci

Molecular determinants of αVβ5 localization in flat clathrin lattices - role of αVβ5 in cell adhesion and proliferation.

Authors - Alba Zuidema
  • KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Lucas (Verified Customer) (08-28-2025)

The antibody is working reasonably in WB, but is not really suitable for IF, at least in the U251-MG cell line. This could be due to a large fraction of soluble MARK2 protein.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:250 (WB), 1:100(IF)
  • Cell Tissue Type: U251-MG
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