Anticorps Polyclonal de lapin anti-MARK2

• Phare
• Validé par KD/KO

MARK2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(16)

Avis (1)

N° de cat : 15492-1-AP

Synonymes

ELKL motif kinase 1, EMK 1, EMK1, MARK2, PAR 1, PAR1 homolog, Par1b

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Galerie de données de validation

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  WB analysis of mouse brain using 15492-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15492-1-AP (MARK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15492-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15492-1-AP (MARK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15492-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15492-1-AP (MARK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 15492-1-AP

  IP result of anti-MARK2 (IP:15492-1-AP, 4ug; Detection:15492-1-AP 1:1000) with rat brain tissue lysate 4000ug.

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  IHC staining of human prostate cancer using 15492-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15492-1-AP (MARK2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15492-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15492-1-AP (MARK2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 15492-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15492-1-AP (MARK2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 15492-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15492-1-AP (MARK2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse testis using 15492-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15492-1-AP (MARK2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15492-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15492-1-AP (MARK2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de rat
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu cérébral humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

15492-1-AP cible MARK2 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MARK2 Protéine recombinante Ag7884
• Nom complet: MAP/microtubule affinity-regulating kinase 2
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 77-90 kDa
• Numéro d’acquisition GenBank: BC008771
• Symbole du gène: MARK2
• Identification du gène (NCBI): 2011
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MARK2 is also named as EMK1(ELKL motif kinase 1), Par1b and belongs to the CAMK Ser/Thr protein kinase family. Par1b/MARK2 plays a critical role in neuronal polarity in the process of axon specification from multiple candidate neurites using primary cultures of mammalian hippocampal neurons(PMID:20194617). It has 16 isoforms produced by alternative promoter usage and alternative splicing with the molecular mass of 77-90kDa and can exsit as a dimer(PMID:16803889).

Publications

Species	Application	Title
human	WB	Mol Cell; An AMPK-Independent Signaling Pathway Downstream of the LKB1 Tumor Suppressor Controls Snail1 and Metastatic Potential.; Authors - Jonathan M Goodwin
human	IP	Nat Commun; TNK1 is a ubiquitin-binding and 14-3-3-regulated kinase that can be targeted to block tumor growth.; Authors - Tsz-Yin Chan ; KD Validated
Mouse	WB,IHC	J Neurosci; Unbiased proteomics of early Lewy body formation model implicates active microtubule affinity-regulating kinases (MARKs) in synucleinopathies.; Authors - Michael X Henderson
		FASEB J; Cdc42 is involved in NC1 peptide-regulated BTB dynamics through actin and microtubule cytoskeletal reorganization.; Authors - Wenhui Su
rat	WB	FASEB J; Regulation of the blood-testis barrier by a local axis in the testis: role of laminin α2 in the basement membrane.; Authors - Ying Gao
human	WB	J Cell Sci; Molecular determinants of αVβ5 localization in flat clathrin lattices - role of αVβ5 in cell adhesion and proliferation.; Authors - Alba Zuidema ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lucas (Verified Customer) (08-28-2025)

The antibody is working reasonably in WB, but is not really suitable for IF, at least in the U251-MG cell line. This could be due to a large fraction of soluble MARK2 protein.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:250 (WB), 1:100(IF)
• Cell Tissue Type: U251-MG

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