Anticorps Polyclonal de lapin anti-MAST3

MAST3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 19507-1-AP

Synonymes

KIAA0561, MAST3

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Galerie de données de validation

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  WB analysis of Jurkat using 19507-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 19507-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 19507-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 19507-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 19507-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 19507-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 19507-1-AP

  IP Result of anti-MAST3 (IP:19507-1-AP, 4ug; Detection:19507-1-AP 1:500) with Jurkat cells lysate 4000ug.

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  IHC staining of human kidney using 19507-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19507-1-AP (MAST3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 19507-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19507-1-AP (MAST3 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 19507-1-AP

  Immunofluorescent analysis of HepG2 cells, using MAST3 antibody 19507-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu pulmonaire de souris
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

19507-1-AP cible MAST3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: microtubule associated serine/threonine kinase 3
• Masse moléculaire calculée: 143 kDa
• Poids moléculaire observé: 144 kDa
• Numéro d’acquisition GenBank: NM_015016
• Symbole du gène: MAST3
• Identification du gène (NCBI): 23031
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAST3, also named as KIAA0561, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. MAST3 catalyze the reaction: ATP + a protein = ADP + a phosphoprotein.