Anticorps Polyclonal de lapin anti-MAT1A/MAT2A

MAT1A/MAT2A Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris, arabidopsis

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 12395-1-AP

Synonymes

AdoMet synthase 1, AMS1, MAT, MAT 1, MAT I/III, MAT1A, MATA1, SAMS, SAMS1

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Galerie de données de validation

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  WB analysis of arabidopsis whole plant using 12395-1-AP

  WB result of MAT1A antibody (12395-1-AP, 1:5000) with plant leaf tissue samples.

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  WB analysis of mouse liver using 12395-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12395-1-AP (MAT1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12395-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12395-1-AP (MAT1A/MAT2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12395-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12395-1-AP (MAT1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12395-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12395-1-AP (MAT1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12395-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12395-1-AP (MAT1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 12395-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12395-1-AP (MAT1A/MAT2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HCT 116, cellules HepG2, tissu hépatique de rat, tissu végétal entier d'arabidopsis
• Résultats positifs en IHC: tissu hépatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

12395-1-AP cible MAT1A/MAT2A dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris, arabidopsis

• Réactivité: Humain, rat, souris, arabidopsis
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAT1A/MAT2A Protéine recombinante Ag3076
• Nom complet: methionine adenosyltransferase I, alpha
• Masse moléculaire calculée: 395 aa, 44 kDa
• Poids moléculaire observé: 44-55 kDa
• Numéro d’acquisition GenBank: BC018359
• Symbole du gène: MAT1A
• Identification du gène (NCBI): 4143
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Methionine adenosyltransferase (MAT) is the enzyme that catalyzes the synthesis of S-adeno-sylmethionine (AdoMet), the main donor of methyl groups in the cell. In mammals MAT is encoded by two different genes, MAT1A and MAT2A. MAT1A is expressed only in the mature liver whereas fetal hepatocytes, extrahepatic tissues and liver cancer cells express MAT2A. This antibody was raised against the full length of human MAT1A and can recognize both of MAT1A and MAT2A. Western blot analysis using this antibody detected a single band around 44-50 kDa in liver.

Publications

Species	Application	Title
human		Ann N Y Acad Sci; PMID: 29722030; Authors - Holly M Brown-Borg
human	WB	Am J Pathol; Forced expression of methionine adenosyltransferase 1A in human hepatoma cells suppresses in vivo tumorigenicity in mice.; Authors - Li Jiaping J
rat	WB	Toxicol Sci; Metabolomic Analysis Reveals Metabolic Changes Caused By Bisphenol A in Rats.; Authors - Minjian Chen
mouse	WB	Proteomics; Early changes in the liver-soluble proteome from mice fed a nonalcoholic steatohepatitis inducing diet.; Authors - Thomas Anja A
mouse	WB	Mech Ageing Dev; Sulfur-based redox alterations in long-lived Snell dwarf mice.; Authors - Vitvitsky Victor V
human	IHC	J Surg Oncol; Histological expression of methionine adenosyl transferase (MAT) 2A as a post-surgical prognostic surrogate in patients with hepatocellular carcinoma.; Authors - Jihyun An

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (03-26-2021)

Great antibody

• Applications: Western Blot
• Cell Tissue Type: Mouse liver, mouse brain / HepG2

MAT1A/MAT2A Antibody Western Blot validation ( dilution) in Mouse liver, mouse brain / HepG2 (Cat no:12395-1-AP)

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