Anticorps Polyclonal de lapin anti-MAT2A

• Phare
• Validé par KD/KO

MAT2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(21)

N° de cat : 55309-1-AP

Synonymes

AMS2, EC:2.5.1.6, MAT 2, MATA2, MATII

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Galerie de données de validation

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  WB analysis using 55309-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55309-1-AP (MAT2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 55309-1-AP

  WB result of MAT2A antibody (55309-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-control and sh-MAT2A transfected HeLa cells.

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  IP experiment of HepG2 using 55309-1-AP

  IP result of anti-MAT2A (IP:55309-1-AP, 4ug; Detection:55309-1-AP 1:500) with HepG2 cells lysate 3200ug.

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  IHC staining of human stomach cancer using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 55309-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 55309-1-AP (MAT2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 55309-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MAT2A antibody (55309-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules NIH/3T3
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer du côlon humain, tissu hépatique de souris, tissu rénal de rat, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Informations sur le produit

55309-1-AP cible MAT2A dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: methionine adenosyltransferase II, alpha
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 47 kDa, 49 kDa
• Numéro d’acquisition GenBank: NM_005911
• Symbole du gène: MAT2A
• Identification du gène (NCBI): 4144
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAT2A, also named as AMS2, MATA2 and AdoMet synthase 2, belongs to the AdoMet synthase family. It catalyzes the formation of S-adenosylmethionine from methionine and ATP. This antibody is specific to MATA2.

Publications

Species	Application	Title
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa ; KD Validated
human	WB,IF	Oxid Med Cell Longev; Oxidative Stress Aggravates Apoptosis of Nucleus Pulposus Cells through m6A Modification of MAT2A Pre-mRNA by METTL16.; Authors - Peng-Bo Chen ; KD Validated
pig	WB	Antioxidants (Basel); M6A RNA Methylation Mediates NOD1/NF-kB Signaling Activation in the Liver of Piglets Challenged with Lipopolysaccharide; Authors - Menghui Xu
mouse	WB	FASEB J; Activating transcription factor 4 regulates angiogenesis under lipid overload via methionine adenosyltransferase 2A-mediated endothelial epigenetic alteration.; Authors - Li Chen ; KD Validated
mouse	WB,IF	FASEB J; Inhibition of MAT2A suppresses osteoclastogenesis and prevents ovariectomy-induced bone loss.; Authors - Honglei Kang ; KD Validated
rat	WB	Environ Toxicol Pharmacol; Ligustrazine modulates renal cysteine biosynthesis in rats exposed to cadmium.; Authors - Wenhua Kuang

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