Anticorps Polyclonal de lapin anti-MATR3

MATR3 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

Avis (2)

N° de cat : 12202-2-AP

Synonymes

KIAA0723, MATR3, matrin 3

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Galerie de données de validation

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  WB analysis of MCF-7 using 12202-2-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12202-2-AP (MATR3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 12202-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12202-2-AP (MATR3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12202-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12202-2-AP (MATR3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12202-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12202-2-AP (MATR3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 12202-2-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using MATR3 antibody (12202-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HEK-293 using 12202-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MATR3 antibody (12202-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7,
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules SH-SY5Y, cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

12202-2-AP cible MATR3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MATR3 Protéine recombinante Ag2843
• Nom complet: matrin 3
• Masse moléculaire calculée: 847 aa, 95 kDa
• Poids moléculaire observé: 125 kDa
• Numéro d’acquisition GenBank: BC015031
• Symbole du gène: MATR3
• Identification du gène (NCBI): 9782
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MATR3, known as KIAA0723, is an 847 amino acid protein, which may play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. MATR3 was originally identified as a nuclear matrix protein containing both DNA and RNA binding domains (PMID: 2033075) with a wide variety of functions ranging from DNA damage response to transcriptional and post-transcriptional regulation of cellular and viral RNAs(PMID: 21771346). the calculated molecular weight of MATR3 is 94 kDa, but we detect a 125 kDa protein as a result of PMID: 28977530.

Publications

Species	Application	Title
human	WB	Nat Commun; TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.; Authors - Roy Matkovic
human	WB	Nat Commun; An oncopeptide regulates m6A recognition by the m6A reader IGF2BP1 and tumorigenesis.; Authors - Song Zhu
human	WB	Brain; MATR3 haploinsufficiency and early-onset neurodegeneration.; Authors - Michael Zech
human	WB	J Cell Biol; Stress granules regulate stress-induced paraspeckle assembly.; Authors - Haiyan An
human	WB	Cell Rep; SATB1 regulates 3D genome architecture in T cells by constraining chromatin interactions surrounding CTCF-binding sites; Authors - Bao Wang
human	WB	RNA Biol; Modulation of PDCD1 exon 3 splicing.; Authors - Junjie Sun

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tatyana (Verified Customer) (03-11-2023)

Good and specific nuclear staining. 4% PFA fixation followed by methanol permeabilization; antibody prepared in 5% goat serum in PBST; 2 h at RT incubation.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human, SH-SY5Y cells

MATR3 Antibody Immunofluorescence validation (1:1000 dilution) in Human, SH-SY5Y cells (Cat no:12202-2-AP)

FH

Wael (Verified Customer) (08-22-2022)

around 50ug of total cell lysate Blocking: 5% skim milk in PBS Primary incubation: 1:1000 in 5% skim milk in PBS overnight, rotating 4C Washes : 3X 15mins of PBS(0.001% Tween) Secondary: 1:10000 in 5% skim milk in PBS, 1hr, RT Washes : 3X 15mins of PBS(0.001% Tween) Specific Band size around 100KD

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hek293

MATR3 Antibody Western Blot validation (1:1000 dilution) in Hek293 (Cat no:12202-2-AP)