Anticorps Polyclonal de lapin anti-MBD2

• Phare
• Validé par KD/KO

MBD2 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 55200-1-AP

Synonymes

Demethylase, DMTase, MBD2, NY CO 41

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Galerie de données de validation

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  WB analysis of Jurkat using 55200-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 55200-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 55200-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 55200-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55200-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55200-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55200-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55200-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 55200-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 55200-1-AP (MBD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 55200-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 55200-1-AP (MBD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A431, cellules HEK-293, tissu cérébral humain
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

55200-1-AP cible MBD2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: methyl-CpG binding domain protein 2
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43-45 kDa
• Numéro d’acquisition GenBank: NM_003927
• Symbole du gène: MBD2
• Identification du gène (NCBI): 8932
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MBD2, also named as DMTase, binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. MBD2 binds hemi-methylated DNA as well. It recruits histone deacetylases and DNA methyltransferases. MBD2 acts as transcriptional repressor and plays a role in gene silencing. (PMID:9774669) This antibody is specific to MBD2. It recognize isoform1 and isoform2 of MBD2.

Publications

Species	Application	Title
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang ; KD Validated
mouse	WB	Exp Cell Res; Loss of MBD2 affects early T cell development by inhibiting the WNT signaling pathway.; Authors - Ling Cheng ; KO Validated
human	WB	Cell Rep; TRPS1 drives heterochromatic origin refiring and cancer genome evolution.; Authors - Jianguo Yang
human	WB	Genet Test Mol Biomarkers; The Expression of MBD6 Is Associated with Tumor Size in Uterine Leiomyomas.; Authors - Mei-Rong Liang
human	WB,IF	Gene; Exploring the importance of m5c in the diagnosis and subtype classification of COPD using the GEO database; Authors - Jianjun Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Manfred (Verified Customer) (05-09-2022)

The antibody worked perfectly without any unspecific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: PBMCs