Anticorps Polyclonal de lapin anti-MBD4

• Phare
• Validé par KD/KO

MBD4 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 11270-1-AP

Synonymes

EC:3.2.2.-, MED1, Methyl-CpG-binding domain protein 4, Methyl-CpG-binding endonuclease 1, Methyl-CpG-binding protein MBD4

---

Galerie de données de validation

• 

  WB analysis of human skin using 11270-1-AP

  human skin tissue were subjected to SDS PAGE followed by western blot with 11270-1-AP (MBD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11270-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11270-1-AP (MBD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: tissu cutané humain, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

11270-1-AP cible MBD4 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MBD4 Protéine recombinante Ag1822
• Nom complet: methyl-CpG binding domain protein 4
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC011752
• Symbole du gène: MBD4
• Identification du gène (NCBI): 8930
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MBD4, also named as MED1, is a 580 amino acid protein, which Interacts with MLH1. MBD4 as a mismatch-specific DNA N-glycosylase is involved in DNA repair and has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. MBD4 can also remove uracil or 5-fluorouracil in G:U mismatches. MBD4 exists some isoforms with MV 66 kDa and 30 kDa (The identification of a novel alternatively spliced form of the MBD4 DNA glycosylase).

Publications

Species	Application	Title
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang ; KD Validated
human	WB	Genet Test Mol Biomarkers; The Expression of MBD6 Is Associated with Tumor Size in Uterine Leiomyomas.; Authors - Mei-Rong Liang

{{ptg:RelatedPrimaryAntibodies}}