Anticorps Recombinant de lapin anti-MBOAT7

MBOAT7 Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240505E8

Avis (1)

N° de cat : 83546-3-RR

Synonymes

h mboa 7, EC:2.3.1.-, Bladder and breast carcinoma-overexpressed gene 1 protein, BB1, 240505E8

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Galerie de données de validation

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  IHC staining of mouse testis using 83546-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83546-3-RR (MBOAT7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 83546-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83546-3-RR (MBOAT7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HuH-7 using 83546-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using MBOAT7 antibody (83546-3-RR, Clone: 240505E8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of A549 using 83546-3-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug MBOAT7 Recombinant antibody (83546-3-RR, Clone:240505E8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83546-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83546-3-RR against Human MBOAT7 were performed. The affinity constant is 0.573 nM.

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Applications testées

• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HuH-7,
• Résultats positifs en FC (Intra): cellules A549,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83546-3-RR cible MBOAT7 dans les applications de IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MBOAT7 Protéine recombinante Ag24466
• Nom complet: membrane bound O-acyltransferase domain containing 7
• Masse moléculaire calculée: 472 aa, 53 kDa
• Numéro d’acquisition GenBank: BC006309
• Symbole du gène: MBOAT7
• Identification du gène (NCBI): 79143
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MBOAT7 (membrane-bound O-acyltransferase domain containing 7), also known as LPIAT, LENG4, or BB1, belongs to the MBOAT superfamily. MBOAT7 acylates lyso-phosphatidylinositol (lyso-PI) with arachidonyl-CoA and is involved in phosphatidylinositol acyl-chain remodeling in the Lands cycle (PMID: 30959108; PMID: 35509994). MBOAT7 deficiency in humans leads to developmental disorders of the central nervous system, with intellectual disability, epilepsy, and autism spectrum disorder (PMID: 37316513). The calculated molecular weight of MBOAT7 is 53 kDa. This antibody can recognize the 45 kDa isoform of MBOAT7.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Eui Jung (Verified Customer) (12-05-2024)

Very good in IFM and validated in KO liver

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: liver

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