Anticorps Polyclonal de lapin anti-CD146/MCAM

• Phare
• Validé par KD/KO

CD146/MCAM Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA, Cell treatment

Conjugaison

Non conjugué

Publications(32)

Avis (1)

N° de cat : 17564-1-AP

Synonymes

CD146, MCAM, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MUC18

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Galerie de données de validation

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  WB analysis of HepG2 using 17564-1-AP

  WB result of CD146/MCAM antibody (17564-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD146/MCAM transfected HepG2 cells.

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  WB analysis using 17564-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17564-1-AP (CD146/MCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17564-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 17564-1-AP (CD146/MCAM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 17564-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17564-1-AP (CD146/MCAM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 17564-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 17564-1-AP (CD146/MCAM antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17564-1-AP

  IP result of anti-CD146/MCAM (IP:17564-1-AP, 4ug; Detection:17564-1-AP 1:1500) with HeLa cells lysate 4000ug.

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  IP experiment of HepG2 using 17564-1-AP

  IP result of anti-CD146/MCAM (IP:17564-1-AP, 3ug; Detection:17564-1-AP 1:500) with HepG2 cells lysate 3600ug.

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  IHC staining of human ovary cancer using 17564-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:40000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 17564-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17564-1-AP (CD146/MCAM antibody) at dilution of 1:40000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using 17564-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD146/MCAM antibody (17564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using 17564-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD146/MCAM antibody (17564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HUVEC using 17564-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CD146/MCAM antibody (17564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A375, cellules HepG2, cellules HUVEC, tissu pulmonaire de souris
• Résultats positifs en IP: cellules HeLa, cellules HepG2
• Résultats positifs en IHC: human ovary cancer tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu splénique de souris,
• Résultats positifs en IF/ICC: cellules HUVEC,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20000-1:80000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 11 publications below
• IHC: See 9 publications below
• IF: See 13 publications below
• IP: See 1 publications below

Informations sur le produit

17564-1-AP cible CD146/MCAM dans les applications de WB, IHC, IF/ICC, IF-P, IP, ELISA, Cell treatment et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, Lapin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD146/MCAM Protéine recombinante Ag11762
• Nom complet: melanoma cell adhesion molecule
• Masse moléculaire calculée: 646 aa, 72 kDa
• Poids moléculaire observé: 113-120 kDa
• Numéro d’acquisition GenBank: BC056418
• Symbole du gène: CD146
• Identification du gène (NCBI): 4162
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

Publications

Species	Application	Title
mouse	IHC	Biomaterials; Macropore design of tissue engineering scaffolds regulates mesenchymal stem cell differentiation fate.; Authors - W Benton Swanson
rat	IF	Adv Healthc Mater; Extrafibrillarly Demineralized Dentin Matrix for Bone Regeneration; Authors - Xiaoyi Wu
human	WB	Oncogene; The histone demethylase KDM3A, and its downstream target MCAM, promote Ewing Sarcoma cell migration and metastasis.; Authors - M Sechler ; KD Validated
human	IF	Cell Prolif; Dental pulp stem cells-based therapy for the oviduct injury via immunomodulation and angiogenesis in vivo.; Authors - Lihua Luo
human		Stem Cell Res Ther; Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis; Authors - Zuping Wu
rat	WB	J Proteome Res; Comparative Proteomic Analysis of Primary Schwann Cells and a Spontaneously Immortalized Schwann Cell Line RSC 96: A Comprehensive Overview with a Focus on Cell Adhesion and Migration Related Proteins.; Authors - Ji Yuhua Y ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (09-28-2020)

This antibody recognises a band around the expected molecular weight, however this band did not respond to deglycosylation treatment. This strongly suggests the recognised band is another protein other than Mcam, because Mcam is glycosylated, and looking at the other Mcam antibody from the same brand (66153-1-g), it is clear that the band is responsive to PNGaseF treatment.

• Applications: Western Blot

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