Anticorps Polyclonal de lapin anti-MCM5

MCM5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(23)

Avis (1)

N° de cat : 11703-1-AP

Synonymes

CDC46, EC:3.6.4.12, P1 CDC46, P1-CDC46

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Galerie de données de validation

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  WB analysis using 11703-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11703-1-AP (MCM5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11703-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11703-1-AP (MCM5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 11703-1-AP

  IP result of anti-MCM5 (IP:11703-1-AP, 4ug; Detection:11703-1-AP 1:4000) with Jurkat cells lysate 2400 ug.

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  IHC staining of mouse thymus using 11703-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11703-1-AP (MCM5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse thymus using 11703-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11703-1-AP (MCM5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 11703-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11703-1-AP (MCM5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 11703-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11703-1-AP (MCM5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11703-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCM5 antibody (11703-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules HeLa, cellules Jurkat, tissu de thymus de rat, tissu de thymus de souris
• Résultats positifs en IP: cellules Jurkat,
• Résultats positifs en IHC: tissu de thymus de souris, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 18 publications below
• IHC: See 6 publications below
• IF: See 1 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

11703-1-AP cible MCM5 dans les applications de WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MCM5 Protéine recombinante Ag2271
• Nom complet: minichromosome maintenance complex component 5
• Masse moléculaire calculée: 82 kDa
• Poids moléculaire observé: 82-88 kDa
• Numéro d’acquisition GenBank: BC000142
• Symbole du gène: MCM5
• Identification du gène (NCBI): 4174
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Minichromosome maintenance, S. Cerevisiae, homolog of 5(MCM5), one component of the complex of MCM2-7, which is the putative replicative essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The MCM complex form a hexameric ring through the interaction surfaces of two neighboring subunits.

Publications

Species	Application	Title
human	WB,RIP,IP	Mol Ther; Long noncoding RNA lnc-POP1-1 upregulated by VN1R5 promotes cisplatin resistance in head and neck squamous cell carcinoma through interaction with MCM5.; Authors - Yingying Jiang
human	WB	Elife; MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cells.; Authors - Yuichiro Saito
human	WB	J Nanobiotechnology; Graphene quantum dots rescue angiogenic retinopathy via blocking STAT3/Periostin/ERK signaling.; Authors - Na Zhao
human	WB	Front Oncol; METTL3 Promotes the Progression of Gastric Cancer via Targeting the MYC Pathway.; Authors - Dong-Dong Yang
human	WB	Oncotarget; Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis.; Authors - Yinan Ma
human	WB	Ecotoxicol Environ Saf; The influence of phenolic environmental estrogen on the transcriptome of uterine leiomyoma cells: A whole transcriptome profiling-based analysis.; Authors - Zemin Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hana (Verified Customer) (07-04-2023)

In this immunoprecipitation experiment, we tested the interaction between MCMBP and MCM5 (detected by 11703-1-AP). One can observe that clear interaction between these two proteins as it was reported previously Sedlackova et al, Nature, 2020.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: human osteosarcoma U2OS

MCM5 Antibody Western Blot,Immunoprecipitation validation (1:2000 dilution) in human osteosarcoma U2OS (Cat no:11703-1-AP)

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