Anticorps Polyclonal de lapin anti-MCM6

• Phare
• Validé par KD/KO

MCM6 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

20

N° de cat : 13347-2-AP

Synonymes

MCG40308, MCM6, Mis5, P105MCM

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Galerie de données de validation

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  WB analysis using 13347-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13347-2-AP (MCM6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13347-2-AP

  WB result of MCM6 antibody (13347-2-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM6 transfected HeLa cells.

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  WB analysis of mouse ovary using 13347-2-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13347-2-AP (MCM6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 13347-2-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13347-2-AP (MCM6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 13347-2-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13347-2-AP (MCM6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13347-2-AP

  IP Result of anti-MCM6 (IP:13347-2-AP, 3ug; Detection:13347-2-AP 1:1000) with HeLa cells lysate 3800ug.

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  IHC staining of human cervical cancer using 13347-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13347-2-AP (MCM6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 13347-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human cervical cancer using 13347-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13347-2-AP (MCM6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 13347-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 13347-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 13347-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 13347-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13347-2-AP (MCM6 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 13347-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13347-2-AP (MCM6 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 13347-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 13347-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 13347-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCM6 antibody (13347-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HeLa, tissu de thymus de souris, tissu ovarien de souris, tissu splénique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 7 publications below
• IF: See 2 publications below

Informations sur le produit

13347-2-AP cible MCM6 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MCM6 Protéine recombinante Ag4180
• Nom complet: minichromosome maintenance complex component 6
• Masse moléculaire calculée: 821 aa, 93 kDa
• Poids moléculaire observé: 105 kDa
• Numéro d’acquisition GenBank: BC032374
• Symbole du gène: MCM6
• Identification du gène (NCBI): 4175
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The MCM genes were firstly identified in yeast defective in minichromosome maintenance and have since been shown to have roles in the progression of the cell cycle, and most of them are cell division control genes[PMID: 18096807]. MCM2-7 complex are suggested to be 'DNA licensing factors' which bind to the DNA after mitosis and enable DNA replication before being removed during S phase. Mini-chromosome maintenance 6 (MCM6) is one component of the MCM2-7 complex which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells[PMID: 9305914]. MCM6 single subunit forms homohexamer and contains an ATP-dependent and replication fork stimulated 3' to 5' DNA unwinding activity along with intrinsic DNA-dependent ATPase and ATP-binding activities [PMID:21336027]. The calculated molecular weight of MCM6 is 92 kDa, but the modified MCM6 is about 105 kDa.

Publications

Species	Application	Title
human	IHC	J Hematol Oncol; Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma; Authors - Yexin Tong
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song
human	WB	Elife; MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cells.; Authors - Yuichiro Saito
human	WB	Front Oncol; METTL3 Promotes the Progression of Gastric Cancer via Targeting the MYC Pathway.; Authors - Dong-Dong Yang
human	WB	Oncotarget; Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis.; Authors - Yinan Ma
human	WB	Ecotoxicol Environ Saf; The influence of phenolic environmental estrogen on the transcriptome of uterine leiomyoma cells: A whole transcriptome profiling-based analysis.; Authors - Zemin Li