Anticorps Polyclonal de lapin anti-MCM8

MCM8 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 16451-1-AP

Synonymes

C20orf154, MCM8, Minichromosome maintenance 8, REC

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Galerie de données de validation

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  WB analysis using 16451-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16451-1-AP (MCM8 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 16451-1-AP

  IP Result of anti-MCM8 (IP:16451-1-AP, 4ug; Detection:16451-1-AP 1:300) with NIH/3T3 cells lysate 4000ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules NIH/3T3
• Résultats positifs en IP: cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

16451-1-AP cible MCM8 dans les applications de WB, IP, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MCM8 Protéine recombinante Ag8696
• Nom complet: minichromosome maintenance complex component 8
• Masse moléculaire calculée: 880 aa, 98 kDa
• Poids moléculaire observé: 95 kDa
• Numéro d’acquisition GenBank: BC008830
• Symbole du gène: MCM8
• Identification du gène (NCBI): 84515
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MCM8, also named as Minichromosome maintenance 8, is a 840 amino acid protein, which belongs to the MCM family. MCM8 is expressed highly in placenta, lung and pancreas and various tumors with highest levels in colon and lung cancers. MCM8 is a component of the MCM8-MCM9 complex, which involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. MCM8 exists some isoforms with MV 94 kDa and 70 kDa.

Publications

Species	Application	Title
human		Nat Commun; DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain.; Authors - Jing Zhang
human	WB	Nat Commun; MCM8IP activates the MCM8-9 helicase to promote DNA synthesis and homologous recombination upon DNA damage.; Authors - Jen-Wei Huang
human	WB	Cell Death Dis; Aberrantly expressed HORMAD1 disrupts nuclear localization of MCM8-MCM9 complex and compromises DNA mismatch repair in cancer cells.; Authors - Kang Liu
human	WB	Res Sq; Mechanism of DNA unwinding by hexameric MCM8-9 in complex with HROB; Authors - Ananya Acharya
human	WB	Nat Commun; A multi-functional role for the MCM8/9 helicase complex in maintaining fork integrity during replication stress; Authors - Wezley C. Griffin
human	WB,IF,CoIP	J Cell Biol; REC drives recombination to repair double-strand breaks in animal mtDNA; Authors - Anna Klucnika