Anticorps Recombinant de lapin anti-MCP-1/CCL2

MCP-1/CCL2 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

230483A9

N° de cat : CL405-98028

Synonymes

Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2

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Galerie de données de validation

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  FC experiment of RAW 264.7 using CL405-98028

  1x10^6 untreated or LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug CoraLite® Plus 405 Anti-Mouse MCP-1 Rabbit Recombinant Antibody (CL405-98028, Clone: 230483A9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of RAW 264.7 using CL405-98028

  1x10^6 LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug CoraLite® Plus 405 Anti-Mouse MCP-1 Rabbit Recombinant Antibody (CL405-98028, Clone: 230483A9) (red) or Isotype Control (blue). 1x10^6 untreated RAW 264.7 cells were intracellularly stained with 0.25 ug CoraLite® Plus 405 Anti-Mouse MCP-1 Rabbit Recombinant Antibody (CL405-98028) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): cellules RAW 264.7 traitées au LPS et à la bréfeldine A,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL405-98028 cible MCP-1/CCL2 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: chemokine (C-C motif) ligand 2
• Numéro d’acquisition GenBank: NM-011333
• Symbole du gène: MCP-1/CCL2
• Identification du gène (NCBI): 20296
• Conjugaison: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 399 nm / 422 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Monocyte chemotactic protein 1 (MCP-1; also known as CCL2), is chemotactic for monocyte/macrophage, B-cell, and T-lymphocyte, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.

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