Anticorps Polyclonal de lapin anti-MCP1

MCP1 Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, FC, Blocking, ELISA

Conjugaison

Non conjugué

Publications

55

N° de cat : 25542-1-AP

Synonymes

C C motif chemokine 2, CCL2, chemokine (C C motif) ligand 2, GDCF 2, HC11, HSMCR30, MCAF, MCP 1, MCP1, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small inducible cytokine A2, SMC CF

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Galerie de données de validation

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  WB analysis using 25542-1-AP

  Untreated THP-1 cells, THP-1 cells (treated with 50 nM PMA and 5 ug/ml LPS for 24 hours, with addition of 300ng/ml Brefeldin A for the last 3 hours.) were subjected to SDS PAGE followed by western blot with 25542-1-AP (MCP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 25542-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25542-1-AP (MCP1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 25542-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25542-1-AP (MCP1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 25542-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25542-1-AP (MCP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 25542-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25542-1-AP (MCP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 25542-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25542-1-AP (MCP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 25542-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25542-1-AP (MCP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of THP-1 using 25542-1-AP

  1X10^6 HP-1 cells untreated (dashed lines) or treated with PMA (red) were intracellularly stained with 0.4 ug Anti-Human MCP1 (25542-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody (Blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: PMA, LPS and Brefeldin A treated THP-1 cells,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du foie humain, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: PMA treated THP-1 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 23 publications below
• IF: See 7 publications below

Informations sur le produit

25542-1-AP cible MCP1 dans les applications de WB, IHC, IF, FC, Blocking, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, Lapin
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MCP1 Protéine recombinante Ag10601
• Nom complet: chemokine (C-C motif) ligand 2
• Masse moléculaire calculée: 99 aa, 11 kDa
• Poids moléculaire observé: 11 kDa
• Numéro d’acquisition GenBank: BC009716
• Symbole du gène: CCL2
• Identification du gène (NCBI): 6347
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Monocyte chemotactic protein 1 (MCP1; also known as CCL2), is a chemokine that can be expressed in monocytes, macrophages, and endothelial cells, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. Research has shown that the expression of MCP1 increases in the serum of patients with acute myocarditis. MCP1 is up-regulated in many types of CNS injury, including ischemia, hemorrhage, trauma, infection, hypoxia, and peripheral nerve axotomy. MCP1 has also been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.

Publications

Species	Application	Title
		ACS Cent Sci; Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury; Authors - Juan Zhang
human		J Neurol Neurosurg Psychiatry; Molecules of senescent glial cells differentiate Alzheimer's disease from ageing; Authors - Linbin Dai
	IF	J Hazard Mater; Polystyrene microplastics induced male reproductive toxicity in mice.; Authors - Haibo Jin
rabbit	IHC	Theranostics; Macrophage targeted theranostic strategy for accurate detection and rapid stabilization of the inflamed high-risk plaque.; Authors - Joon Woo Song
human	IHC	Theranostics; Reversing activity of cancer associated fibroblast for staged glycolipid micelles against internal breast tumor cells.; Authors - Yun Zhu
	IHC	Int J Cancer; Immunogenomic analysis reveals LGALS1 contributes to the immune heterogeneity and immunosuppression in glioma.; Authors - Qun Chen

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