Anticorps Polyclonal de lapin anti-MCT1

• Phare
• Validé par KD/KO

MCT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

57

Avis (3)

N° de cat : 20139-1-AP

Synonymes

HHF7, MCT, MCT 1, MCT1, Monocarboxylate transporter 1, SLC16A1

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Galerie de données de validation

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  WB analysis using 20139-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20139-1-AP (MCT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20139-1-AP

  WB result of MCT1 antibody (20139-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCT1 transfected HeLa cells.

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  WB analysis of mouse brain using 20139-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20139-1-AP (MCT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 20139-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20139-1-AP (MCT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 20139-1-AP

  IP result of anti-MCT1 (IP:20139-1-AP, 4ug; Detection:20139-1-AP 1:300) with Raji cells lysate 3600 ug.

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  IHC staining of human colon cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 20139-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 20139-1-AP (MCT1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 20139-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MCT1 antibody (20139-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 20139-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MCT1 antibody (20139-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HeLa, cellules HepG2, cellules Jurkat, tissu cérébral de souris, tissu hépatique de souris
• Résultats positifs en IP: cellules Raji,
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du col de l'utérus humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:3000-1:20000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 41 publications below
• IHC: See 17 publications below
• IF: See 8 publications below
• FC: See 1 publications below

Informations sur le produit

20139-1-AP cible MCT1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MCT1 Protéine recombinante Ag14098
• Nom complet: solute carrier family 16, member 1 (monocarboxylic acid transporter 1)
• Masse moléculaire calculée: 500 aa, 54 kDa
• Poids moléculaire observé: 38-45 kDa
• Numéro d’acquisition GenBank: BC026317
• Symbole du gène: SLC16A1
• Identification du gène (NCBI): 6566
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MCT1, the SLC16A1 gene product, is a trans-membrane symporter involved in lactate and pyruvate transportation. It plays an important role in lactic acid transport and H+ clearance in cancer cells. MCT overexpression had been observed in various cancers and may play an important role in tumorigenesis. Two isoforms of MCT1 exist due to the alternative splicing, with predicted MW of 54 kDa and 46 kDa, respectively. While western blot analysis detected MCT1 at an apparent molecular mass of 40-50 kDa.

Publications

Species	Application	Title
mouse	WB,IHC	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
mouse	WB	Nat Cancer; Targeting the bicarbonate transporter SLC4A4 overcomes immunosuppression and immunotherapy resistance in pancreatic cancer; Authors - Federica Cappellesso
mouse	IHC	Clin Cancer Res; A Novel Role for DNA-PK in Metabolism by Regulating Glycolysis in Castration Resistant Prostate Cancer.; Authors - Emanuela Dylgjeri
human	WB,IHC	Theranostics; ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.; Authors - Xiaoxin Zhang
rat	WB	Acta Pharmacol Sin; Pinocembrin attenuates hemorrhagic transformation after delayed t-PA treatment in thromboembolic stroke rats by regulating endogenous metabolites.; Authors - Ling-Lei Kong
human	WB,IHC	Haematologica; Inhibition of monocarboxylate transporter 1 by AZD3965 as a novel therapeutic approach for the treatment of diffuse large B-cell lymphoma and Burkitt lymphoma.; Authors - Richard A Noble

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xie (Verified Customer) (07-07-2023)

The expression of MCT4 was seen in my samples.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: brain

MCT1 Antibody Immunofluorescence validation (1:250 dilution) in brain (Cat no:20139-1-AP)

FH

Aderonke (Verified Customer) (05-23-2023)

The expression of MCT4 was seen in three of the five cell lines used without an inhibitor. Also, an inhibtor of MCT4, was used at two different concentrations and the expression was decreased in the cells that expressed the protein.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10,000
• Cell Tissue Type: Head and Neck cancer cell lines

FH

Huseyin (Verified Customer) (07-28-2022)

I used the antibody with 1:3000 dilution in TBS-T solution O/N and I received correct size bands in Ishikawa cell line.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Ishikawa Cell Line