Anticorps Polyclonal de lapin anti-MCT1

• Phare
• Validé par KD/KO

MCT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(98)

Avis (4)

N° de cat : 20139-1-AP

Synonymes

SLC16A1, FLJ36745, HHF7, MCT, MCT 1

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Galerie de données de validation

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  WB analysis of HeLa using 20139-1-AP

  WB result of MCT1 antibody (20139-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCT1 transfected HeLa cells.

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  WB analysis of BxPC-3 using 20139-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 20139-1-AP (MCT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 20139-1-AP

  IP result of anti-MCT1 (IP:20139-1-AP, 4ug; Detection:20139-1-AP 1:300) with Raji cells lysate 3600 ug.

• 

  IHC staining of human colon cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 20139-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20139-1-AP (MCT1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 20139-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MCT1 antibody (20139-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HeLa
• Résultats positifs en IP: cellules Raji,
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du col de l'utérus humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:600-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 69 publications below
• IHC: See 28 publications below
• IF: See 26 publications below
• IP: See 1 publications below

Informations sur le produit

20139-1-AP cible MCT1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MCT1 Protéine recombinante Ag14098
• Nom complet: solute carrier family 16, member 1 (monocarboxylic acid transporter 1)
• Masse moléculaire calculée: 500 aa, 54 kDa
• Poids moléculaire observé: 38-45 kDa
• Numéro d’acquisition GenBank: BC026317
• Symbole du gène: MCT1
• Identification du gène (NCBI): 6566
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MCT1, the SLC16A1 gene product, is a trans-membrane symporter involved in lactate and pyruvate transportation. It plays an important role in lactic acid transport and H+ clearance in cancer cells. MCT overexpression had been observed in various cancers and may play an important role in tumorigenesis. Two isoforms of MCT1 exist due to the alternative splicing, with predicted MW of 54 kDa and 46 kDa, respectively. While western blot analysis detected MCT1 at an apparent molecular mass of 40-50 kDa.

Publications

Species	Application	Title
mouse	WB,IHC	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
mouse	IF	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
mouse	WB	Nat Cancer; Targeting the bicarbonate transporter SLC4A4 overcomes immunosuppression and immunotherapy resistance in pancreatic cancer; Authors - Federica Cappellesso
human	WB	J Exp Clin Cancer Res; Lnc AC016727.1/BACH1/HIF-1 α signal loop promotes the progression of non-small cell lung cancer; Authors - Li Zhang
mouse	IHC	Clin Cancer Res; A Novel Role for DNA-PK in Metabolism by Regulating Glycolysis in Castration Resistant Prostate Cancer.; Authors - Emanuela Dylgjeri
human	WB,IHC	Theranostics; ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.; Authors - Xiaoxin Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Angie (Verified Customer) (08-27-2024)

A549 lysate was subjected to western blot with MCT1 antibody used at 1:10000 dilution and incubated at room temperature for 1.5 hours. Secondary antibody Donkey-anti-rabbit (Alexa Fluor 800) used at 1:20000 dilution incubated at room temperature for 1 hour. One thick band at around 40 kDa. Image is shown in grayscale.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: A549

MCT1 Antibody Western Blot validation (1:10000 dilution) in A549 (Cat no:20139-1-AP)

FH

Xie (Verified Customer) (07-07-2023)

The expression of MCT4 was seen in my samples.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: brain

MCT1 Antibody Immunofluorescence validation (1:250 dilution) in brain (Cat no:20139-1-AP)

FH

Aderonke (Verified Customer) (05-23-2023)

The expression of MCT4 was seen in three of the five cell lines used without an inhibitor. Also, an inhibtor of MCT4, was used at two different concentrations and the expression was decreased in the cells that expressed the protein.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10,000
• Cell Tissue Type: Head and Neck cancer cell lines

FH

Huseyin (Verified Customer) (07-28-2022)

I used the antibody with 1:3000 dilution in TBS-T solution O/N and I received correct size bands in Ishikawa cell line.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Ishikawa Cell Line

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