Anticorps Polyclonal de lapin anti-MDH2

MDH2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(25)

Avis (1)

N° de cat : 15462-1-AP

Synonymes

EC:1.1.1.37, MDH, MDH 2

---

Galerie de données de validation

• 

  WB analysis using 15462-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15462-1-AP (MDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15462-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15462-1-AP (MDH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15462-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15462-1-AP (MDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15462-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15462-1-AP (MDH2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 15462-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 15462-1-AP (MDH2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 15462-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15462-1-AP (MDH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15462-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15462-1-AP (MDH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 15462-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15462-1-AP (MDH2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15462-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15462-1-AP (MDH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15462-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15462-1-AP (MDH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 15462-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MDH2 antibody (15462-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, tissu pulmonaire de rat, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 23 publications below
• IP: See 1 publications below

Informations sur le produit

15462-1-AP cible MDH2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris, Caenorhabditis elegans
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MDH2 Protéine recombinante Ag7750
• Nom complet: malate dehydrogenase 2, NAD (mitochondrial)
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC001917
• Symbole du gène: MDH2
• Identification du gène (NCBI): 4191
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MDH2 encodes the Krebs cycle enzyme mitochondrial malate dehydrogenase, which catalyzes the reversible oxidation of malate to oxaloacetate and plays a role in the malate-aspartate NADH shuttle. Mutations in MDH2 Cause Developmental and epileptic encephalopathy 51 (PMID: 27989324).

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma; Authors - Qifan Hu
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang
human	WB	J Exp Med; Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.; Authors - Miao Liu
mouse	WB	Aging Dis; Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging; Authors - Minghao Yuan
mouse	WB	iScience; The Symbiotic Relationship between the Neural Retina and Retinal Pigment Epithelium Is Supported by Utilizing Differential Metabolic Pathways.; Authors - Tirthankar Sinha
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Genome-wide translational reprogramming of genes important for myocyte functions in overload-induced heart failure.; Authors - Qianqian Guo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Süleyman (Verified Customer) (02-06-2022)

It is good antibody but some unspecific bands, but MDH2 can be detected specifically around 34 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

MDH2 Antibody Western Blot validation (1:1000 dilution) in HeLa (Cat no:15462-1-AP)

{{ptg:RelatedPrimaryAntibodies}}