Anticorps Polyclonal de lapin anti-Midkine

• Phare
• Validé par KD/KO

Midkine Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 11009-1-AP

Synonymes

ARAP, MDK, Midkine, MK, MK1, NEGF2

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Galerie de données de validation

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  WB analysis using 11009-1-AP

  Mouse embryo tissue were subjected to SDS PAGE followed by western blot with 11009-1-AP (Midkine antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 11009-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11009-1-AP (Midkine antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 11009-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11009-1-AP (Midkine antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse embryo using 11009-1-AP

  IP result of anti-Midkine(IP:11009-1-AP, 4ug; Detection:11009-1-AP 1:3000) with mouse embryo tissue lysate 4800 ug.

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  IHC staining of human liver cancer using 11009-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11009-1-AP (Midkine antibody at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 11009-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Midkine antibody (11009-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu embryonnaire de souris, cellules COLO 320, cellules SH-SY5Y
• Résultats positifs en IP: tissu embryonnaire de souris,
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

11009-1-AP cible Midkine dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Midkine Protéine recombinante Ag1465
• Nom complet: midkine (neurite growth-promoting factor 2)
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC011704
• Symbole du gène: MDK
• Identification du gène (NCBI): 4192
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Midkine is a heparin-binding growth factor identified over 20 years ago and enhances the survival, migration and many other activities of target cells. Midkine is rich in both basic amino acids and cysteine, and is not related to most other growth factors/cytokines. It is strongly expressed during embryonic periods, especially at the midgestation stage, and plays important roles in development, especially in neurogenesis. Midkine expression in adult tissue is generally weak or undetectable, and it is induced upon injury and exerts many activities related to tissue repair. The biological activities of midkine in malignant tumors include proliferation, angiogenesis, invasion and metastasis. Various cancers express significantly higher levels of the midkine protein in early stage tumor tissues than in adjacent normal tissue.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.; Authors - Federica Morani
human	WB,IHC,IF	Front Immunol; Roles of the m6A Modification of RNA in the Glioblastoma Microenvironment as Revealed by Single-Cell Analyses.; Authors - Feng Yuan ; KD Validated
human	WB	Front Oncol; Targeting MDK Abrogates IFN-γ-Elicited Metastasis inCancers of Various Origins.; Authors - Luyu Zheng
human	WB	Cancer Cell Int; Midkine promotes glioblastoma progression via PI3K-Akt signaling.; Authors - Beiquan Hu ; KD Validated
		Am J Respir Cell Mol Biol; Mesenchymal Glucocorticoid Receptor Regulates Development of Multiple Cell Layers of the Mouse Lung.; Authors - Bird A Daniel AD
rat	IHC	J Mol Neurosci; Electroacupuncture Alleviated Neuronal Apoptosis Following Ischemic Stroke in Rats via Midkine and ERK/JNK/p38 Signaling Pathway.; Authors - Ying Xing