Anticorps Polyclonal de lapin anti-MDM2/HDM2

• Phare
• Validé par KD/KO

MDM2/HDM2 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(37)

N° de cat : 27883-1-AP

Synonymes

MDM2, E3 ubiquitin-protein ligase Mdm2, EC:2.3.2.27, hdm2, p53-binding protein Mdm2

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Galerie de données de validation

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  WB analysis using 27883-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27883-1-AP (MDM2/HDM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 27883-1-AP

  WB result of MDM2/HDM2 antibody (27883-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MDM2/HDM2 transfected A431 cells.

• 

  WB analysis using 27883-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27883-1-AP (MDM2/HDM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U2OS using 27883-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MG132 treated U2OS cells using MDM2/HDM2 antibody (27883-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 27883-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human MDM2/HDM2 (27883-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules A431, cellules A549, cellules HepG2, cellules T-47D
• Résultats positifs en IF/ICC: MG132 treated U2OS cells,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 35 publications below
• IHC: See 4 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 4 publications below

Informations sur le produit

27883-1-AP cible MDM2/HDM2 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MDM2/HDM2 Protéine recombinante Ag27255
• Nom complet: Mdm2 p53 binding protein homolog (mouse)
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 75-85 kDa, 57-60 kDa, 40-55 kDa
• Numéro d’acquisition GenBank: NM_001145337.2
• Symbole du gène: MDM2
• Identification du gène (NCBI): 4193
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

What is the cellular localization of MDM2?

MDM2 is predominantly localized in the nucleoplasm.

What is the tissue specificity of MDM2?

MDM2 is non-specific, expressing ubiquitously in the brain, heart, kidney, skin, and other tissues. Certain 

isoforms only express in cancerous cells and not normal tissue.

With what other molecules does MDM2 interact?

Most notably, the oncogene protein MDM2 regulates transcription factor p53 by binding its transcriptional 

activation domain and by E3-ubiquitin ligase activity. Ribosome proteins rpL5, rpL11, and rpL23 also bind to 

MDM2, inhibiting E3 ubiquitin ligase activity and promoting the p53 pathway.

What competes with MDM2 activity?

Nutilin-3 was designed to compete with MDM2 in binding with p53, inducing activation of the p53 pathway.

What is the role of ubiquitination in MDM2 activity?

While p53 is also affected by other post-translational modifications, its stability is determined by ubiquitination 

mediation of MDM2, suppressing p53 transcriptional regulation and leading to the proteosomal degradation of 

p53.

How is MDM2 associated with cancer?

MDM2 inhibits the activity of the tumor-suppressive protein p53, which serves as an important factor in 

promoting DNA repair or activating apoptotic pathways. Loss of p53 is associated with tumor development 

and growth.

What is the human ortholog of MDM2?

Human double-minute type 2 (HDM2)

In what cellular circumstances does the p53-MDM2 complex dissociate?

Cellular stress or DNA damage result in the phosphorylation and activation of p53. Phosphorylation also 

prevents p53-MDM2 interaction.

Where is the gene located?

Hdm2 is located on human 12q15.

Mdm2 is located on murine 10 D2.

PMIDs: 24309898, 29584707, 9840926, 28714398

Publications

Species	Application	Title
rat	WB	J Clin Invest; ZFP36L2 suppresses mTORc1 through a P53-dependent pathway to prevent peri-partum cardiomyopathy in mice.; Authors - Hidemichi Kouzu
mouse,human	WB,IF	Cell Chem Biol; Repurposing AS1411 for constructing ANM-PROTACs; Authors - Xuekun Fu
mouse	WB	Mol Metab; Multi-Omics after O-GlcNAc Alteration Identified Cellular Processes Promoting Aneuploidy after Loss of O-GlcNAc Transferase; Authors - Samuel S Boyd
human	WB,IF,CoIP	Eur J Med Chem; Development of hybrid aptamers-engineered PROTACs for degrading VEGF165 in both tumor- and vascular endothelial cells; Authors - Ziting Feng
		PLoS Pathog; Pseudorabies virus inhibits progesterone-induced inactivation of TRPML1 to facilitate viral entry; Authors - Bing-Qian Su
human	WB	Bioorg Chem; Design, synthesis and biological evaluation of novel pyrrolidone-based derivatives as potent p53-MDM2 inhibitors.; Authors - Dongjuan Si

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