Anticorps Polyclonal de lapin anti-ME1

• Phare
• Validé par KD/KO

ME1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(22)

Avis (1)

N° de cat : 16619-1-AP

Synonymes

EC:1.1.1.40, Malic enzyme 1, NADP-dependent malic enzyme, NADP-ME

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Galerie de données de validation

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  WB analysis using 16619-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 16619-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16619-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16619-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16619-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16619-1-AP (ME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 16619-1-AP

  IP result of anti-ME1 (IP:16619-1-AP, 4ug; Detection:16619-1-AP 1:2000) with mouse liver tissue lysate 2400 ug.

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  IHC staining of human liver using 16619-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16619-1-AP (ME1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16619-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16619-1-AP (ME1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 16619-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16619-1-AP (ME1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 16619-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16619-1-AP (ME1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16619-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16619-1-AP (ME1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16619-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16619-1-AP (ME1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 16619-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ME1 antibody (16619-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, tissu hépatique de rat, tissu hépatique de souris, tissu placentaire de souris
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu hépatique humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 17 publications below
• IHC: See 7 publications below
• IF: See 1 publications below

Informations sur le produit

16619-1-AP cible ME1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ME1 Protéine recombinante Ag9916
• Nom complet: malic enzyme 1, NADP(+)-dependent, cytosolic
• Masse moléculaire calculée: 572 aa, 64 kDa
• Poids moléculaire observé: 55-64 kDa
• Numéro d’acquisition GenBank: BC025246
• Symbole du gène: ME1
• Identification du gène (NCBI): 4199
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ME1(NADP-dependent malic enzyme) belongs to the malic enzymes family. This malic enzyme catalyzes the reversible oxidative decarboxylation of malate and is a link between the glycolytic pathway and the citric acid cycle. The reaction is L-malate plus NADP(+) to form pyruvate, CO(2), and NADPH. The predicted protein contains 572 amino acids and has a calculated molecular mass of 64.1 kDa.

Publications

Species	Application	Title
human	WB	Cancer Cell; Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming.; Authors - Yoichiro Mitsuishi
mouse	WB,IHC	Adv Sci (Weinh); Malic Enzyme 1 as a Novel Anti-Ferroptotic Regulator in Hepatic Ischemia/Reperfusion Injury; Authors - Xuexian Fang
		Mol Cell; Dynamic Regulation of ME1 Phosphorylation and Acetylation Affects Lipid Metabolism and Colorectal Tumorigenesis.; Authors - Yahui Zhu
human	WB	Cell Res; cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.; Authors - Linchong Sun
mouse	WB	Cell Rep; Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism; Authors - Hao Qin
	WB	Cell Rep; Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutation; Authors - Zehe Ge

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chi (Verified Customer) (10-17-2024)

I have found the antibody in a publication (https://pubmed.ncbi.nlm.nih.gov/22789539/) and have tested the antibody myself. The antibody works great in WB with no background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Liver cancer

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