Anticorps Polyclonal de lapin anti-MED10

MED10 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 15196-1-AP

Synonymes

L6, MED10, mediator complex subunit 10, NUT2, TRG 17, TRG 20, TRG17, TRG20

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Galerie de données de validation

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  WB analysis of COLO 320 using 15196-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15196-1-AP (MED10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules COLO 320

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

15196-1-AP cible MED10 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MED10 Protéine recombinante Ag7336
• Nom complet: mediator complex subunit 10
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC003353
• Symbole du gène: MED10
• Identification du gène (NCBI): 84246
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MED10, also named as L6, TRG17, or TRG20, is a 135 amino acid protein, which belongs to the Mediator complex subunit 10 family. MED10 localizes in the nucleus and as a component of the Mediator complex, a coactivator is involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Multiprotein mediator complex acting as a coactivator is required for activation of RNA polymerase II transcription by DNA bound transcription factors.

Publications

Species	Application	Title
human	WB	J Virol; Adenovirus L-E1A activates transcription through mediator complex-dependent recruitment of the super elongation complex.; Authors - Vijayalingam S S

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

SITING (Verified Customer) (04-02-2021)

can see band aroun 15kd

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: EMBRYO HEART

MED10 Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:15196-1-AP)

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