Anticorps Polyclonal de lapin anti-MED30

• Phare
• Validé par KD/KO

MED30 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

Avis (1)

N° de cat : 16787-1-AP

Synonymes

MED30, mediator complex subunit 30, THRAP6, TRAP25

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Galerie de données de validation

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  WB analysis of human heart using 16787-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16787-1-AP (MED30 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16787-1-AP

  WB result of MED30 antibody (16787-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-MED30 transfected HEK-293 cells.

• 

  IP experiment of mouse heart using 16787-1-AP

  IP result of anti-MED30 (IP:16787-1-AP, 4ug; Detection:16787-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, cellules HEK-293
• Résultats positifs en IP: tissu cardiaque de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 4 publications below

Informations sur le produit

16787-1-AP cible MED30 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MED30 Protéine recombinante Ag10221
• Nom complet: mediator complex subunit 30
• Masse moléculaire calculée: 178 aa, 20 kDa
• Poids moléculaire observé: 20-25 kDa
• Numéro d’acquisition GenBank: BC008226
• Symbole du gène: MED30
• Identification du gène (NCBI): 90390
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MED30, also named as THRAP6 and TRAP25, is a component of the Mediator complex. MED30 has two isoforms with MW 16-20 kDa ad 20-25 kDa.

Publications

Species	Application	Title
human	IHC	J Autoimmun; Immune complexome analysis of a rich variety of serum immune complexes identifies disease-characteristic immune complex antigens in systemic sclerosis; Authors - Yuki Jimbayashi Kutsuna
human	WB, IHC	PLoS One; MED30 Regulates the Proliferation and Motility of Gastric Cancer Cells.; Authors - Yong Joo Lee
human	WB,IHC	Anticancer Res; The Contrasting Role of the Mediator Subunit MED30 in the Progression of Bladder Cancer.; Authors - Isabella Syring ; KD Validated
human	WB,IHC	Ann Diagn Pathol; The knockdown of the mediator complex subunit MED30 suppresses the proliferation and migration of renal cell carcinoma cells.; Authors - Isabella Syring ; KD Validated
		Nat Genet; Interaction between MED12 and ΔNp63 activates basal identity in pancreatic ductal adenocarcinoma; Authors - Diogo Maia-Silva

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tongbin (Verified Customer) (08-25-2020)

This antibody detects a MED30 band at around 22kD.

• Applications: Western Blot
• Cell Tissue Type: Heart

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