Anticorps Polyclonal de lapin anti-MED31

MED31 Polyclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF,ELISA

Conjugaison

Non conjugué

Publications

1

Avis (2)

N° de cat : 16590-1-AP

Synonymes

3110004H13Rik, CGI 125, hSOH1, MED31, mediator complex subunit 31, Mediator complex subunit SOH1, Soh1

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Galerie de données de validation

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  WB analysis of mouse testis using 16590-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16590-1-AP (MED31 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16590-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16590-1-AP (MED31 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 16590-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MED31 antibody (16590-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu cérébral humain
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

16590-1-AP cible MED31 dans les applications de WB, IF,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MED31 Protéine recombinante Ag9008
• Nom complet: mediator complex subunit 31
• Masse moléculaire calculée: 131 aa, 16 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC012539
• Symbole du gène: MED31
• Identification du gène (NCBI): 51003
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MED31, also named as SOH1 or CGI-125, is a 131 amino acid protein, which belongs to the Mediator complex subunit 31 family. MED31 localizes in the nucleus. MED31 as a component of the Mediator complex, a coactivator is involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED31 functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.

Publications

Species	Application	Title
mouse	WB	Dev Biol; The Mediator complex protein Med31 is required for embryonic growth and cell proliferation during mammalian development.; Authors - Risley Michael D MD

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Anh (Verified Customer) (07-15-2021)

very clear bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse cardiomyocytes

FH

siting (Verified Customer) (02-17-2021)

good band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse heart

MED31 Antibody Western Blot validation (1:1000 dilution) in mouse heart (Cat no:16590-1-AP)