Anticorps Polyclonal de lapin anti-MEF2C

MEF2C Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 16953-1-AP

Synonymes

myocyte enhancer factor 2C

---

Galerie de données de validation

• 

  WB analysis of human heart using 16953-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16953-1-AP (MEF2C antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of TF-1 using 16953-1-AP

  TF-1 cells were subjected to SDS PAGE followed by western blot with 16953-1-AP (MEF2C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16953-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16953-1-AP (MEF2C antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 16953-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16953-1-AP (MEF2C antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: tissu cardiaque humain, cellules HeLa, cellules TF-1, tissu de muscle squelettique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

16953-1-AP cible MEF2C dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MEF2C Protéine recombinante Ag10583
• Nom complet: myocyte enhancer factor 2C
• Masse moléculaire calculée: 469 aa, 51 kDa
• Poids moléculaire observé: 45-70 kDa
• Numéro d’acquisition GenBank: BC026341
• Symbole du gène: MEF2C
• Identification du gène (NCBI): 4208
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development[PMID: 20221419]. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission[PMID:18599438]. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis[PMID: 21666133]. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. MEF2C exists some isoforms with MV 50-52 kDa, 47 kDa, and 45 kDa, but modified MEF2C is about 55-66 kDa.

{{ptg:RelatedPrimaryAntibodies}}