Anticorps Polyclonal de lapin anti-MEF2D

• Phare
• Validé par KD/KO

MEF2D Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

Avis (1)

N° de cat : 14353-1-AP

Synonymes

MEF2D, myocyte enhancer factor 2D

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Galerie de données de validation

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  WB analysis of C2C12 using 14353-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 14353-1-AP (MEF2D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14353-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14353-1-AP (MEF2D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 14353-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14353-1-AP (MEF2D antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14353-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14353-1-AP (MEF2D antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14353-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14353-1-AP (MEF2D antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellule C2C12, cellules HeLa
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

14353-1-AP cible MEF2D dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MEF2D Protéine recombinante Ag5700
• Nom complet: myocyte enhancer factor 2D
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC040949
• Symbole du gène: MEF2D
• Identification du gène (NCBI): 4209
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MEF2D, also named as Myocyte-specific enhancer factor 2D, is a 521 amino acid protein, which belongs to the MEF2 family. MEF2D is expressed in t in myotubes and also in undifferentiated myoblasts. MEF2D as a transcriptional activator which binds specifically to the MEF2 element, found in numerous muscle-specific and stress-induced genes. MEF2D mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. The calculated molecular weight of MEF2D is 55 kDa, but Phosphorylated MEF2D is 60 kDa.

Publications

Species	Application	Title
mouse	WB,IF	Nat Commun; Alternatively spliced exon regulates context-dependent MEF2D higher-order assembly during myogenesis; Authors - Mónika Gönczi
human	WB	Autophagy; MEF2D-NR4A1-FAM134B2-mediated reticulophagy contributes to amino acid homeostasis; Authors - Yuji Shiozaki
rat	WB	Ecotoxicol Environ Saf; GPX4 degradation via chaperone-mediated autophagy contributes to antimony-triggered neuronal ferroptosis.; Authors - Shali Yu
human	WB,IF	J Cell Mol Med; DYRK1A phosphorylates MEF2D and decreases its transcriptional activity.; Authors - Pin Wang
mouse	WB,IHC	J Transl Med; Extracellular vesicle miR-32 derived from macrophage promotes arterial calcification in mice with type 2 diabetes via inhibiting VSMC autophagy.; Authors - Jingsong Cao
mouse	WB	J Cell Physiol; PMID: 30488495; Authors - Jiali Nie

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chao (Verified Customer) (03-12-2020)

Weak but predicted size band in adult mouse heart.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart