Anticorps Polyclonal de lapin anti-MEK1-Specific

MEK1-Specific Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 51080-1-AP

Synonymes

ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK 1, MEK1, MEK1-Specific, MKK1, PRKMK1

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Galerie de données de validation

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  IHC staining of human endometrial cancer using 51080-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 51080-1-AP (MEK1-Specific antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human endometrial cancer using 51080-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 51080-1-AP (MEK1-Specific antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 51080-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 51080-1-AP (MEK1-Specific antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 51080-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 51080-1-AP (MEK1-Specific antibody at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu de cancer de l'endomètre humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below

Informations sur le produit

51080-1-AP cible MEK1-Specific dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris, Eriocheir Sinensis
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mitogen-activated protein kinase kinase 1
• Masse moléculaire calculée: 43 kDa
• Numéro d’acquisition GenBank: BC139729
• Symbole du gène: MEK1
• Identification du gène (NCBI): 5604
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MEK1 is also named as MAP2K1 (mitogen-activated protein kinase kinase 1), MAPKK1, PRKMK1, MKK1 and belongs to the MAP kinase kinase subfamily. It is a dual-specificity kinase that mediate ERK1 and ERK2 activation during adhesion and growth factor signaling(PMID:19219045). It also plays an essential role in extra-embryonic ectoderm during placentogenesis. MEK1 has 2 isoforms produced by alternative splicing with the molecular weight of 43 kDa and 41 kDa. It can be phosphorylated(PMID:21144847). This antibody is specific to MEK1.

Publications

Species	Application	Title
mouse		J Ethnopharmacol; Classical prescription Floris Sophorae Powder treat colorectal cancer by regulating KRAS/MEK-ERK signaling pathway; Authors - Li-Ying Han
human	WB	Cancer Cell Int; MiR-155-5p suppresses SOX1 to promote proliferation of cholangiocarcinoma via RAF/MEK/ERK pathway.; Authors - Da Wang
human	WB	Cancer Cell Int; 4,5-Dimethoxycanthin-6-one is a novel LSD1 inhibitor that inhibits proliferation of glioblastoma cells and induces apoptosis and pyroptosis.; Authors - Wei Li
human	WB	Mol Cell Probes; Elevated PGT promotes proliferation and inhibits cell apoptosis in preeclampsia by Erk signaling pathway; Authors - Huiyuan Pang
human	WB	PLoS One; Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis.; Authors - Xu Jiang
human	WB	Onco Targets Ther; FANCI Cooperates with IMPDH2 to Promote Lung Adenocarcinoma Tumor Growth via a MEK/ERK/MMPs Pathway.; Authors - Pengchao Zheng

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