Anticorps Recombinant de lapin anti-c-Met (Cytoplasmic)

c-Met (Cytoplasmic) Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

2G12

N° de cat : 82904-1-RR

Synonymes

c-Met, MET, HGF receptor, EC:2.7.10.1, c Met

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Galerie de données de validation

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  WB analysis of HepG2 using 82904-1-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 82904-1-RR (c-Met antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82904-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82904-1-RR (c-Met antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 82904-1-RR

  WB result of c-Met (Cytoplasmic) antibody (82904-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-c-Met (Cytoplasmic) transfected HepG2 cells.

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  WB analysis of HEK-293 using 82904-1-RR

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 82904-1-RR (c-Met antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 82904-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug c-Met (Cytoplasmic) Recombinant antibody (82904-1-RR, Clone:2G12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82904-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82904-1-RR against Human c-Met (Cytoplasmic) were performed. The affinity constant is 0.573 nM.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82904-1-RR cible c-Met (Cytoplasmic) dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: c-Met (Cytoplasmic) Protéine recombinante Ag23140
• Nom complet: met proto-oncogene (hepatocyte growth factor receptor)
• Masse moléculaire calculée: 1390 aa, 155 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC130420
• Symbole du gène: MET
• Identification du gène (NCBI): 4233
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

c-Met (also named MET or HGFR) is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to the HGF ligand. c-Met regulates many physiological processes including proliferation, scattering, morphogenesis, and survival. The primary single-chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide-linked to form the mature receptor. Overexpression and/or mutation of c-Met has been reported in various human malignancies, including lung cancer, breast cancer, head and neck cancer, gastric cancer, colorectal cancer, bladder cancer, uterine cervix carcinoma, esophageal carcinoma, c-Met could serve as an important therapeutic target (PMID: 26036285). The c-met receptor is a 190-kD glycoprotein consisting of a 145-kD membrane-spanning beta chain and a 50-kD alpha chain (PMID: 7806559). In Western blot, this antibody produces bands of unknown identity at 55 and 100 kDa.

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