Anticorps Polyclonal de lapin anti-METTL1

• Phare
• Validé par KD/KO

METTL1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

19

Avis (2)

N° de cat : 14994-1-AP

Synonymes

C12orf1, methyltransferase like 1, METTL1, TRM8, tRNA(m7G46) methyltransferase, YDL201w

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Galerie de données de validation

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  WB analysis using 14994-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14994-1-AP (METTL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14994-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14994-1-AP (METTL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of A2780 using 14994-1-AP

  IP result of anti-METTL1 (IP:14994-1-AP, 4ug; Detection:14994-1-AP 1:300) with A2780 cells lysate 3600 ug.

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  IHC staining of human liver cancer using 14994-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14994-1-AP (METTL1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14994-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 14994-1-AP (METTL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HuH-7, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules A2780,
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 15 publications below
• WB: See 16 publications below
• IHC: See 9 publications below
• IF: See 1 publications below

Informations sur le produit

14994-1-AP cible METTL1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: METTL1 Protéine recombinante Ag6980
• Nom complet: methyltransferase like 1
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC000550
• Symbole du gène: METTL1
• Identification du gène (NCBI): 4234
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

METTL1 methyltransferase mediates m7G methylation within miRNAs and regulates cell migration via its catalytic activity. METTL1 can be inactivated by phosphorylation at Ser27 by protein kinase B (PKBα). Overexpression of METTL1 is widely observed among human cancers. It is also crucial for the regulation of chemoresistance in cancer treatment. In addition, mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation.

Publications

Species	Application	Title
mouse	WB	Nature; Structural basis of regulated m7G tRNA modification by METTL1-WDR4; Authors - Jiazhi Li ; KO Validated
human	WB	Cell Res; Dynamic methylome of internal mRNA N7-methylguanosine and its regulatory role in translation.; Authors - Lionel Malbec
human,mouse	WB,IHC	Mol Cell; N7-Methylguanosine tRNA modification enhances oncogenic mRNA translation and promotes intrahepatic cholangiocarcinoma progression.; Authors - Zihao Dai ; KD Validated
human,mouse	WB	Mol Cell; METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation.; Authors - Esteban A Orellana ; KD Validated
mouse		Mol Cell; Mettl1/Wdr4-Mediated m7G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation.; Authors - Shuibin Lin ; KO Validated
human,mouse	IHC	Nat Commun; N7-methylguanosine tRNA modification promotes esophageal squamous cell carcinoma tumorigenesis via the RPTOR/ULK1/autophagy axis.; Authors - Hui Han ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Zhongwen (Verified Customer) (09-25-2023)

Clear band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

METTL1 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:14994-1-AP)

FH

Hannah (Verified Customer) (12-05-2018)

Single specific band detected at expected size after overnight primary incubation.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: human fibroblast

METTL1 Antibody Western Blot, validation (1/1000 dilution) in human fibroblast (Cat no:14994-1-AP)