Anticorps Polyclonal de lapin anti-METTL14

• Phare
• Validé par KD/KO

METTL14 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(157)

Avis (2)

N° de cat : 26158-1-AP

Synonymes

hMETTL14, Methyltransferase-like protein 14, METT L14, N6-adenosine-methyltransferase non-catalytic subunit

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Galerie de données de validation

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  WB analysis of Raji using 26158-1-AP

  WB result of METTL14 antibody (26158-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL14 transfected Raji cells.

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  WB analysis using 26158-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26158-1-AP (METTL14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 26158-1-AP

  IP result of anti-METTL14 (IP:26158-1-AP, 4ug; Detection:26158-1-AP 1:2000) with A549 cells lysate 880 ug.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules NCCIT, cellules PC-3
• Résultats positifs en IP: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 32 publications below
• WB: See 127 publications below
• IHC: See 21 publications below
• IF: See 18 publications below
• IP: See 2 publications below
• CoIP: See 3 publications below
• RIP: See 15 publications below

Informations sur le produit

26158-1-AP cible METTL14 dans les applications de WB, IHC, IF, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc, poulet, singe
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: METTL14 Protéine recombinante Ag14325
• Nom complet: methyltransferase like 14
• Masse moléculaire calculée: 456 aa, 52 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC007449
• Symbole du gène: METTL14
• Identification du gène (NCBI): 57721
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

METTL14, is also named as Methyltransferase-like protein 14 or KIAA1627, is a 456 amino acid protein, which belongs to the MT-A70-like family and localized in the nucleus. The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues of some mRNAs and regulates the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability. M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naïve pluripotency-promoting transcripts results in transcript destabilization.

Publications

Species	Application	Title
	WB	Cell Metab; Amelioration of nonalcoholic fatty liver disease by inhibiting the deubiquitylating enzyme RPN11; Authors - Bing Zhou
human	WB	Cell Res; Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.; Authors - Fei Peng
human	IF	Cell Res; The m6A methylome of SARS-CoV-2 in host cells.; Authors - Jun'e Liu ; KD Validated
	WB,RIP	Nat Cell Biol; The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA.; Authors - Xiang-Qian Gao
human	WB	Nat Commun; Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma; Authors - Weiwei Dai
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

RASHMI (Verified Customer) (07-26-2024)

used this product, highly recommended

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK-293

FH

RASHMI (Verified Customer) (07-26-2024)

Used this product, highly recommended

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK-293

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