Anticorps Recombinant de lapin anti-METTL3

• Phare
• Validé par KD/KO

METTL3 Recombinant Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

2I9

N° de cat : 80323-1-RR

Synonymes

IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8

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Galerie de données de validation

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  WB analysis using 80323-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80323-1-RR (METTL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 80323-1-RR

  WB result of METTL3 antibody (80323-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL3 transfected HeLa cells.

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  IHC staining of human lung cancer using 80323-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 80323-1-RR (METTL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 80323-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80323-1-RR (METTL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 80323-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 80323-1-RR (METTL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 80323-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80323-1-RR (METTL3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 80323-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80323-1-RR (METTL3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 80323-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80323-1-RR (METTL3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 80323-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80323-1-RR (METTL3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 80323-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80323-1-RR (METTL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 80323-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80323-1-RR (METTL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 80323-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80323-1-RR (METTL3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 80323-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80323-1-RR (METTL3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 80323-1-RR

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 80323-1-RR (METTL3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 80323-1-RR

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 80323-1-RR (METTL3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 80323-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 80323-1-RR (METTL3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 80323-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 80323-1-RR (METTL3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 80323-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80323-1-RR (METTL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 80323-1-RR

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 80323-1-RR (METTL3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 80323-1-RR

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 80323-1-RR (METTL3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Jurkat using 80323-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using METTL3 antibody (80323-1-RR, Clone: 2I9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules Neuro-2a, cellules NIH/3T3
• Résultats positifs en IHC: tissu testiculaire de souris, tissu de cancer de l'œsophage humain, tissu de cancer du côlon humain, tissu de cancer du poumon humain, tissu testiculaire de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

80323-1-RR cible METTL3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: METTL3 Protéine recombinante Ag7110
• Nom complet: methyltransferase like 3
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC001650
• Symbole du gène: METTL3
• Identification du gène (NCBI): 56339
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.