Anticorps Polyclonal de lapin anti-METTL6

• Phare
• Validé par KD/KO

METTL6 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 16527-1-AP

Synonymes

EC:2.1.1.-, hMETTL6, methyltransferase like 6, Methyltransferase-like protein 6, tRNA N(3)-methylcytidine methyltransferase METTL6

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Galerie de données de validation

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  WB analysis using 16527-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16527-1-AP (METTL6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16527-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16527-1-AP (METTL6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 16527-1-AP

  IP result of anti-METTL6 (IP:16527-1-AP, 4ug; Detection:16527-1-AP 1:5000) with MCF-7 cells lysate 1600 ug.

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  IHC staining of human lung cancer using 16527-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16527-1-AP (METTL6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 16527-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16527-1-AP (METTL6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16527-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16527-1-AP (METTL6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 16527-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16527-1-AP (METTL6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 16527-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16527-1-AP (METTL6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 16527-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16527-1-AP (METTL6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary using 16527-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16527-1-AP (METTL6 antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules MCF-7
• Résultats positifs en IP: cellules MCF-7,
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu cérébral humain, tissu cutané humain, tissu de cancer du sein humain, tissu ovarien humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

16527-1-AP cible METTL6 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: METTL6 Protéine recombinante Ag9768
• Nom complet: methyltransferase like 6
• Masse moléculaire calculée: 255 aa, 30 kDa
• Poids moléculaire observé: 30-32 kDa
• Numéro d’acquisition GenBank: BC022400
• Symbole du gène: METTL6
• Identification du gène (NCBI): 131965
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Methyltransferase-like protein 6 (METTL6) belongs to the METL family. METTL6 is as a crucial regulator of tumor cell growth, and METTL6 is a bona fide transfer RNA (tRNA) methyltransferase, catalyzing the formation of 3-methylcytidine at C32 of specific serine tRNA isoacceptors (PMID:32923617). METTL6 is a member of the RNA methyltransferase family that has been identified in many cancers. The mRNA expression levels of METTL6 were significantly upregulated in HCC tumor tissues compared to that in adjacent non tumor tissues. CRISPR/Cas9 mediated knockout of METTL6 in hepatocellular carcinoma cell lines remarkably inhibited colony formation, cell proliferation, cell migration, cell invasion and cell attachment ability (PMID:34913069).

Publications

Species	Application	Title
human	IHC	Front Immunol; Identification and validation of a tyrosine metabolism-related prognostic prediction model and characterization of the tumor microenvironment infiltration in hepatocellular carcinoma; Authors - Yangying Zhou
human	WB	J Biol Chem; Three distinct 3-methylcytidine (m(3)C) methyltransferases modify tRNA and mRNA in mice and humans.; Authors - Luang Xu ; KO Validated
human	WB	Nat Commun; m3C32 tRNA modification controls serine codon-biased mRNA translation, cell cycle, and DNA-damage response; Authors - Jia Cui ; KO Validated

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