Anticorps Monoclonal anti-MFN1

MFN1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

CloneNo.

3F11C11

Avis (1)

N° de cat : 66776-1-Ig

Synonymes

DKFZp762F247, FLJ20693, Fzo homolog, hfzo1, hfzo2, MFN1, mitofusin 1, Transmembrane GTPase MFN1

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Galerie de données de validation

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  WB analysis using 66776-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66776-1-Ig (MFN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66776-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66776-1-Ig (MFN1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 66776-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66776-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66776-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66776-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66776-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MFN1 antibody (66776-1-Ig, Clone: 3F11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules MG U-87, cellules NIH/3T3, cellules PC-3, cellules U-251
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

66776-1-Ig cible MFN1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MFN1 Protéine recombinante Ag4890
• Nom complet: mitofusin 1
• Masse moléculaire calculée: 741 aa, 84 kDa
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC040557
• Symbole du gène: MFN1
• Identification du gène (NCBI): 55669
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria. Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).

Publications

Species	Application	Title
human	IF	Nat Commun; BNIP3L/NIX-mediated mitophagy protects against glucocorticoid-induced synapse defects.; Authors - Gee Euhn Choi
pig	IF	Ecotoxicol Environ Saf; Zinc deficiency compromises the maturational competence of porcine oocyte by inducing mitophagy and apoptosis; Authors - Xin-Le Lai
human	WB	Molecules; Nujiangexanthone A Inhibits Cervical Cancer Cell Proliferation by Promoting Mitophagy.; Authors - Jiling Feng
rat	IHC	J Mol Histol; Roles of HIF-1α/BNIP3 mediated mitophagy in mitochondrial dysfunction of letrozole-induced PCOS rats; Authors - Fan Wang
human,rat	WB	Phytomedicine; Xiangsha Liujunzi Decoction improves gastrointestinal motility in functional dyspepsia with spleen deficiency syndrome by restoring mitochondrial quality control homeostasis; Authors - Jiaqi Zhang
human	WB	J Nutr Biochem; miR-494-5p mediates the antioxidant activity of EPA by targeting the mitochondrial elongation factor 1 gene MIEF1 in HepG2 cells; Authors - Yanqin Lin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

benjamin (Verified Customer) (01-31-2019)

Very strong for WB with 20ug of protein using heLa cells

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HeLa PWPI