Anticorps Polyclonal de lapin anti-MZB1

MZB1 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF

Conjugaison

Non conjugué

Publications

7

N° de cat : 11454-1-AP

Synonymes

hypothetical protein MGC29506, MZB1, PACAP, pERp1

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Galerie de données de validation

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  WB analysis of Raji using 11454-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11454-1-AP (MZB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 11454-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11454-1-AP (MZB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 11454-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11454-1-AP (MZB1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human small intestine using 11454-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine slide using 11454-1-AP (MZB1 Antibody) at dilution of 1:50.

• 

  IHC staining of human small intestine using 11454-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine slide using 11454-1-AP (MZB1 Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: cellules Raji
• Résultats positifs en IHC: tissu de gliome humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 6 publications below
• IF: See 2 publications below

Informations sur le produit

11454-1-AP cible MZB1 dans les applications de WB, IHC, IF et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MZB1 Protéine recombinante Ag2015
• Nom complet: hypothetical protein MGC29506
• Masse moléculaire calculée: 189 aa, 21 kDa
• Poids moléculaire observé: 19-21 kDa
• Numéro d’acquisition GenBank: BC021275
• Symbole du gène: MGC29506
• Identification du gène (NCBI): 51237
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MZB1, also named as ,PACAP, MGC29506 and pERp1, belongs to the PERP1 family. It is associates with immunoglobulin M (IgM) heavy and light chains and promotes IgM assembly and secretion. MGC29506(PACAP) may exert its effect by acting as a molecular chaperone or as an oxidoreductase as it displays a low level of oxidoreductase activity. Isoform 2 may be involved in regulation of apoptosis. (PubMed: 11350957) MZB1 has 5 isoforms with MW 21kd and 7-13kd.

Publications

Species	Application	Title
human	IHC	Clin Cancer Res; Integrative array-based approach identifies MZB1 as a frequently methylated putative tumor suppressor in hepatocellular carcinoma.; Authors - Matsumura Satoshi S
human	IHC,IF	J Pathol; Proteomic profiling identifies novel diagnostic biomarkers and molecular subtypes for mucinous tubular and spindle cell carcinoma of the kidney.; Authors - Huiya Xu
human	IHC	Int J Cancer; Epigenetic suppression of the immunoregulator MZB1 is associated with the malignant phenotype of gastric cancer.; Authors - Mitsuro Kanda
human	WB	J Biol Chem; Transgenic expression of microRNA-185 causes a developmental arrest of T cells by targeting multiple genes including Mzb1.; Authors - Belkaya Serkan S
human	WB,IHC,IF	Arthritis Res Ther; Increase of MZB1 in B cells in systemic lupus erythematosus: proteomic analysis of biopsied lymph nodes.; Authors - Aya Miyagawa-Hayashino
human	IHC	Oncol Lett; MZB1 expression indicates poor prognosis in estrogen receptor-positive breast cancer.; Authors - Manabu Watanabe

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