Anticorps Monoclonal anti-MGP

• Phare
• Validé par KD/KO

MGP Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(7)

CloneNo.

1A1C3

N° de cat : 60055-1-Ig

Synonymes

GIG36, 1A1C3, Cell growth-inhibiting gene 36 protein, matrix Gla protein, MGLAP

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Galerie de données de validation

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  WB analysis using 60055-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 60055-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 60055-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human saliva using 60055-1-Ig

  human saliva were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver cancer using 60055-1-Ig

  human liver cancer tissue were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60055-1-Ig (MGP antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 10x lens).

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  IHC staining of human gliomas using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 40x lens).

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  IHC staining of human heart using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 10x lens).

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  IHC staining of human heart using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 40x lens).

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  IHC staining of human kidney using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 10x lens).

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  IHC staining of human kidney using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 40x lens).

• 

  IHC staining of human brain using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human gliomas using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 60055-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse kidney using 60055-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using MGP antibody (60055-1-Ig, Clone: 1A1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  Affinity and Kinetic Characterization of 60055-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 60055-1-Ig against Human MGP were performed. The Affinity Constant is 5.42 nM.

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, salive humain, salive humaine, tissu cérébral humain, tissu de cancer du foie humain, tissu rénal humain
• Résultats positifs en IHC: tissu de gliome humain, tissu cardiaque humain, tissu cérébral humain, tissu de cancer du côlon humain, tissu pulmonaire humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu rénal de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

60055-1-Ig cible MGP dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MGP Protéine recombinante Ag1091
• Nom complet: matrix Gla protein
• Masse moléculaire calculée: 103 aa, 13 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC005272
• Symbole du gène: MGP
• Identification du gène (NCBI): 4256
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Matrix Gla protein (MGP) is is a vitamin K-dependent, extracellular matrix protein. MGP plays a pivotal role in preventing soft tissue calcification and local mineralization of the vascular wall. Vitamin K deficiency leads to inactive uncarboxylated MGP (ucMGP), which accumulates at sites of arterial calcification. However MGP is synthesized in many tissues and is especially enriched in embryonic tissues and in cancer cells. Defects in MGP are the cause of Keutel syndrome (KS), which is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia.

Publications

Species	Application	Title
human	WB	J Inflamm Res; circ_TGFBR2 Inhibits Vascular Smooth Muscle Cells Phenotypic Switch and Suppresses Aortic Dissection Progression by Sponging miR-29a; Authors - Zhenjun Xu
human	WB	J Biol Chem; Reduced APPL1 impairs osteogenic differentiation of mesenchymal stem cells by facilitating MGP expression to disrupt the BMP2 pathway in osteoporosis; Authors - Weiquan Yuan ; KD Validated
human	WB,IF	Int Immunopharmacol; MGP+ and IDO1+ tumor-associated macrophages facilitate immunoresistance in breast cancer revealed by single-cell RNA sequencing; Authors - Kexin Chang
mouse	IHC	Adv Sci (Weinh); Mgp High-Expressing MSCs Orchestrate the Osteoimmune Microenvironment of Collagen/Nanohydroxyapatite-Mediated Bone Regeneration; Authors - Zhuqing Wan
	IF	Nat Commun; Decoding the mechanical characteristics of the human anterior cruciate ligament entheses through graduated mineralization interfaces; Authors - Jinghua Fang
mouse	IHC	Mol Ther; Dysregulation of Astrocyte-derived Matrix Gla Protein Impairs Dendritic Spine Development in Pyridoxine-dependent Epilepsy; Authors - Junjie Wu

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