Anticorps Polyclonal de lapin anti-RNF156

• Phare
• Validé par KD/KO

RNF156 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (1)

N° de cat : 11285-1-AP

Synonymes

KIAA0544, mahogunin, ring finger 1, MGRN1, RING finger protein 156, RNF156

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Galerie de données de validation

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  WB analysis using 11285-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11285-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11285-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11285-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11285-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11285-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11285-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11285-1-AP (RNF156 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11285-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11285-1-AP (RNF156 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11285-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11285-1-AP (RNF156 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11285-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11285-1-AP (RNF156 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 11285-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11285-1-AP (RNF156 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

11285-1-AP cible RNF156 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNF156 Protéine recombinante Ag1808
• Nom complet: mahogunin, ring finger 1
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 58-64 kDa
• Numéro d’acquisition GenBank: BC014199
• Symbole du gène: MGRN1
• Identification du gène (NCBI): 23295
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MGRN1(E3 ubiquitin-protein ligase) mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. It also plays a role in the regulation of endosome-to-lysosome trafficking. Human and mouse mahogunin exhibit a similar expression pattern across multiple tissues with particularly high levels in brain(PMID: 12560552). It has 4 isoforms produced by alternative splicing with the molecular mass of 58-64 kDa.

Publications

Species	Application	Title
human	WB,IF	Mol Cell; Aging Triggers Cytoplasmic Depletion and Nuclear Translocation of the E3 Ligase Mahogunin: A Function for Ubiquitin in Neuronal Survival.; Authors - Stefano Benvegnù ; KD Validated
human	WB	Dev Cell; A Membrane-Tethered Ubiquitination Pathway Regulates Hedgehog Signaling and Heart Development.; Authors - Jennifer H Kong
human	WB	Pigment Cell Melanoma Res; MGRN1-dependent pigment-type switching requires its ubiquitination activity but not its interaction with TSG101 or NEDD4.; Authors - Gunn Teresa M TM
human,mouse	IP	Development; Gene-teratogen interactions influence the penetrance of birth defects by altering Hedgehog signaling strength.; Authors - Jennifer H Kong
human	WB,IHC	Front Oncol; Promoter Methylation of the MGRN1 Gene Predicts Prognosis and Response to Chemotherapy of High-Grade Serous Ovarian Cancer Patients.; Authors - Xiao-Fei Li
mouse	WB,IF	J Biol Chem; Mahoganoid and mahogany mutations rectify the obesity of the yellow mouse by effects on endosomal traffic of MC4R protein.; Authors - Overton John D JD ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Parijat (Verified Customer) (09-20-2023)

Works well.

• Applications: Western Blot