Anticorps Polyclonal de lapin anti-MICALL2

• Phare
• Validé par KD/KO

MICALL2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(5)

Avis (1)

N° de cat : 24408-1-AP

Synonymes

JRAB, MICAL L2, MICAL like 2, MICAL like protein 2, MICALL2

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Galerie de données de validation

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  WB analysis of PC-3 using 24408-1-AP

  WB result of MICALL2 antibody (24408-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MICALL2 transfected PC-3 cells.

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  WB analysis using 24408-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24408-1-AP (MICALL2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 24408-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 24408-1-AP (MICALL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules HeLa, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

24408-1-AP cible MICALL2 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MICALL2 Protéine recombinante Ag19931
• Nom complet: MICAL-like 2
• Masse moléculaire calculée: 904 aa, 98 kDa
• Poids moléculaire observé: 109 kDa
• Numéro d’acquisition GenBank: BC037988
• Symbole du gène: MICALL2
• Identification du gène (NCBI): 79778
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IHC	Int J Mol Sci; Comprehensive Analysis of MICALL2 Reveals Its Potential Roles in EGFR Stabilization and Ovarian Cancer Cell Invasion; Authors - Tianxiang Xia ; KD Validated
human	WB,IHC,IF	Biochem Pharmacol; MICAL-L2, as an estrogen-responsive gene, is involved in ER-positive breast cancer cell progression and tamoxifen sensitivity via the AKT/mTOR pathway; Authors - Pushuai Wen ; KD Validated
human	WB,IHC,IF,CoIP	Biochim Biophys Acta Mol Basis Dis; High MICAL-L2 promotes cancer progression and drug resistance in renal clear cell carcinoma cells through stabilization of ACTN4 following vimentin expression; Authors - Weizhen Zhao ; KD Validated
human	WB,IHC,IF,IP	Cell Commun Signal; MICALL2 as a substrate of ubiquitinase TRIM21 regulates tumorigenesis of colorectal cancer; Authors - Pushuai Wen ; KD Validated
mouse	IF	Arch Biochem Biophys; MICALL2 participates in the regulation of epithelial-mesenchymal transition in alveolar epithelial cells - Potential roles in pulmonary fibrosis; Authors - Yingying Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joleen (Verified Customer) (05-04-2019)

WB required long exposure >100s. Multiple background bands. Molecular weight is BioRad Precision Plus.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

MICALL2 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:24408-1-AP)

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