Anticorps Polyclonal de lapin anti-MITF

MITF Polyclonal Antibody for FC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

26

Avis (1)

N° de cat : 13092-1-AP

Synonymes

bHLHe32, MI, MITF, WS2A

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Galerie de données de validation

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  WB analysis using 13092-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13092-1-AP (MITF antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skin using 13092-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 13092-1-AP (MITF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 13092-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13092-1-AP (MITF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13092-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13092-1-AP (MITF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 13092-1-AP

  IP Result of anti-MITF (IP:13092-1-AP, 4ug; Detection:13092-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

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  FC experiment of hESC cells using 13092-1-AP

  FC result of anti-MITF (13092-1-AP, 1:50) with hESC which spontaneous differentiation toward retinal lineage (RPE). (Black: control; Red MITF).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules Jurkat, cellules NIH/3T3, tissu cardiaque de souris, tissu cutané de rat
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en cytométrie: cellules SCEh

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC): FC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 23 publications below
• IF: See 3 publications below

Informations sur le produit

13092-1-AP cible MITF dans les applications de WB, IP, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MITF Protéine recombinante Ag3679
• Nom complet: microphthalmia-associated transcription factor
• Masse moléculaire calculée: 91 aa, 10 kDa, 59 kDa
• Poids moléculaire observé: 59-65 kDa
• Numéro d’acquisition GenBank: BC012503
• Symbole du gène: MITF
• Identification du gène (NCBI): 4286
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The retinal pigment epithelium (RPE) has a essential role in maintaining visual function and dedifferentiation of RPE contributes to the pathophysiology of several ocular diseases[PMID: 22523078]. Microphthalmia-associated transcription factor (MITF) is a key regulator of RPE differentiation that is also down-regulated in dedifferentiated hfRPE cells. MITF is a basic helix-loop-helix (hHLH)-leucine zipper protein that involves in the development of various cell types, including neural crest-derived melanocytes and optic cup-derived retinal pigment epithelial cells [PMID: 10578055].

Publications

Species	Application	Title
human	IF	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang
mouse	WB	J Clin Invest; mTORC1 feedback to AKT modulates lysosomal biogenesis through MiT/TFE regulation.; Authors - Kaushal Asrani
mouse	WB	Theranostics; TFE3, a potential therapeutic target for Spinal Cord Injury via augmenting autophagy flux and alleviating ER stress.; Authors - Kailiang Zhou
human,mouse	WB	Phytomedicine; Taxifolin inhibits melanoma proliferation/migration impeding USP18/Rac1/JNK/β-catenin oncogenic signaling; Authors - Libo Xu
human, mouse	WB	J Invest Dermatol; PMID: 23223141; Authors - Lee Chang Seok CS
human	WB	Pigment Cell Melanoma Res; D-tyrosine negatively regulates melanin synthesis by competitively inhibiting tyrosinase activity.; Authors - Jisu Park

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alessandro (Verified Customer) (11-06-2022)

no unspecific staining, great outcome

• Applications: Immunofluorescence