Anticorps Polyclonal de lapin anti-AF-6

AF-6 Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 55102-1-AP

Synonymes

AF 6, AF6, AFADIN, Afadin variant, MLLT4

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Galerie de données de validation

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  WB analysis of mouse kidney using 55102-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55102-1-AP (AF-6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 55102-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55102-1-AP (AF-6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 55102-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55102-1-AP (AF-6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules A431, tissu cérébral humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

55102-1-AP cible AF-6 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4
• Masse moléculaire calculée: 207 kDa
• Poids moléculaire observé: 182-210 kDa
• Numéro d’acquisition GenBank: NM_005936
• Symbole du gène: MLLT4
• Identification du gène (NCBI): 4301
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: NaHCO3 0,1 M, glycine 0,1 M, azoture de sodium à 0,02 % et glycérol à 50 %, pH 7,3.
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MLLT4, also named as AF6 and Afadin, belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). It is a nNectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton. The antibody is specific to MLLT4.

Publications

Species	Application	Title
mouse	WB	J Ethnopharmacol; Wuzi-Yanzong-Wan prevents oligoasthenospermia due to TAp73 suppression by affecting cellular junction remodeling in testicular tissue in mice; Authors - Deling Wu
rat	WB	Res Sq; Novel Populations of Lung Capillary Endothelial Cells and Their Functional Significance; Authors - Joel James
mouse	WB	Aging (Albany NY); The mechanism of simultaneous intake of Jujuboside A and B in the regulation of sleep at the hypothalamic level; Authors - Wei Wang