Anticorps Polyclonal de lapin anti-MLXIPL

MLXIPL Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 13256-1-AP

Synonymes

bHLHd14, CHREBP, MIO, MLX interacting protein like, MLX interactor, MLXIPL, MONDOB, WBSCR14, WS bHLH

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Galerie de données de validation

• 

  WB analysis of HepG2 using 13256-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13256-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13256-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13256-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13256-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13256-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13256-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13256-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13256-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13256-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13256-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13256-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13256-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13256-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13256-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 13256-1-AP

  IP Result of anti-MLXIPL (IP:13256-1-AP, 4ug; Detection:13256-1-AP 1:1000) with HepG2 cells lysate 3600ug.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, tissu hépatique humain
• Résultats positifs en IP: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

13256-1-AP cible MLXIPL dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MLXIPL Protéine recombinante Ag3481
• Nom complet: MLX interacting protein-like
• Masse moléculaire calculée: 852 aa, 93 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC012925
• Symbole du gène: MLXIPL
• Identification du gène (NCBI): 51085
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Carbohydrate response element-binding protein (ChREBP), also known as MLXIPL, is a glucose-responsive transcription factor that has a fundamental role in the glucose-mediated induction of genes involved in hepatic glycolysis and lipogenesis. Circulating blood glucose levels affect ChREBP activity in hepatocytes largely by post-translational mechanisms that include phosphorylation-dependent subcellular localization [PMID:21665952]. It enhances expression of the liver pyruvate kinase (LPK) gene and all lipogenic enzyme genes resulting in the conversion of excess dietary carbohydrate into triglycerides that can be stored as fat [PMID:15118080]. Molecular Weight of ChREBP splice variants: 62/78/91/93 kDa.

Publications

Species	Application	Title
mouse	WB,IHC	Clin Transl Med; Phosphoproteome reveals molecular mechanisms of aberrant rhythm in neurotransmitter-mediated islet hormone secretion in diabetic mice.; Authors - Yunqiang He
mouse		Front Pharmacol; Roxadustat, a Hypoxia-Inducible Factor 1α Activator, Attenuates Both Long- and Short-Term Alcohol-Induced Alcoholic Liver Disease.; Authors - Yongyao Gao
mouse,human	WB	J Agric Food Chem; Diosmetin Ameliorates Nonalcoholic Steatohepatitis through Modulating Lipogenesis and Inflammatory Response in a STAT1/CXCL10-Dependent Manner.; Authors - Nanxuan Luo
human,mouse	WB	J Agric Food Chem; Baicalein Enhances the Effect of Acarbose on the Improvement of Nonalcoholic Fatty Liver Disease Associated with Prediabetes via the Inhibition of De Novo Lipogenesis.; Authors - Yan Xing
human	WB	Food Funct; Polysaccharide MCP extracted from Morchella esculenta reduces atherosclerosis in LDLR-deficient mice.; Authors - Dandan Wang
mouse	WB	Front Pharmacol; PMID: 31427967; Authors - Xin Qiao