Anticorps Polyclonal de lapin anti-MME,CD10

MME,CD10 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC

Conjugaison

Non conjugué

Publications

2

N° de cat : 23898-1-AP

Synonymes

Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11

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Galerie de données de validation

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  WB analysis of mouse kidney using 23898-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (MME,CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 23898-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (MME,CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 23898-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (MME,CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 23898-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (MME,CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 23898-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23898-1-AP (MME,CD10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 23898-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23898-1-AP (MME,CD10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 23898-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using MME,CD10 antibody (23898-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 23898-1-AP

  Immunofluorescent analysis of HepG2 cells, using MME antibody 23898-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 23898-1-AP

  1X10^6 HepG2 cells were stained with .2ug MME,CD10 antibody (23898-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu rénal de rat, tissu rénal humain
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu rénal de souris, cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

23898-1-AP cible MME,CD10 dans les applications de WB, IHC, IF, FC et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MME,CD10 Protéine recombinante Ag20963
• Nom complet: membrane metallo-endopeptidase
• Masse moléculaire calculée: 750 aa, 86 kDa
• Poids moléculaire observé: 90-110 kDa
• Numéro d’acquisition GenBank: BC101658
• Symbole du gène: MME
• Identification du gène (NCBI): 4311
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

Publications

Species	Application	Title
human	IF	Cell Physiol Biochem; NR4A1 is Involved in Fibrogenesis in Ovarian Endometriosis.; Authors - Xinliu Zeng
mouse	WB	Biochem Cell Biol; IL-6 induced enhanced clearance of proANP and ANP by insulin-degrading enzyme in T1DM mouse.; Authors - Caiyun Li