Anticorps Polyclonal de lapin anti-Neprilysin/CD10

Neprilysin/CD10 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 23898-1-AP

Synonymes

MME, MME,CD10, EPN, Enkephalinase, EC:3.4.24.11

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Galerie de données de validation

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  WB analysis of mouse kidney using 23898-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 23898-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 23898-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 23898-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 23898-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 23898-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23898-1-AP (Neprilysin/CD10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 23898-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Neprilysin/CD10 antibody (23898-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of Ramos using 23898-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Ramos cells using MME,CD10 antibody (23898-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu rénal de rat, tissu rénal humain
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu rénal de souris
• Résultats positifs en IF/ICC: cellules Ramos,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

23898-1-AP cible Neprilysin/CD10 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Neprilysin/CD10 Protéine recombinante Ag20963
• Nom complet: membrane metallo-endopeptidase
• Masse moléculaire calculée: 750 aa, 86 kDa
• Poids moléculaire observé: 90-110 kDa
• Numéro d’acquisition GenBank: BC101658
• Symbole du gène: CD10
• Identification du gène (NCBI): 4311
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).

Publications

Species	Application	Title
human	IF	Cell Physiol Biochem; NR4A1 is Involved in Fibrogenesis in Ovarian Endometriosis.; Authors - Xinliu Zeng
mouse	WB	Biochem Cell Biol; IL-6 induced enhanced clearance of proANP and ANP by insulin-degrading enzyme in T1DM mouse.; Authors - Caiyun Li
human	WB	J Biochem Mol Toxicol; miRNA-211-5p inhibition enhances the protective effect of hucMSC-derived exosome in Aβ1-40 -induced SH-SY5Y cells by increasing NEP expression; Authors - Huijing Chen
human	IF	J Transl Med; Lysine acetyltransferase 14 mediates TGF-β-induced fibrosis in ovarian endometrioma via co-operation with serum response factor; Authors - Yi Gong

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