Anticorps Monoclonal anti-Neprilysin/CD10

• Phare
• Validé par KD/KO

Neprilysin/CD10 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

4B9D9

N° de cat : 60034-3-Ig

Synonymes

MME, Neprilysin, 4B9D9, Atriopeptidase, CALLA

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Galerie de données de validation

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  WB analysis using 60034-3-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 60034-3-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60034-3-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60034-3-Ig

  WB result of Neprilysin/CD10 antibody (60034-3-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Neprilysin/CD10 transfected HEK-293 cells.

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  WB analysis of Ramos using 60034-3-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig kidney using 60034-3-Ig

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 60034-3-Ig

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:100000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:100000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:100000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:100000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsil using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsil using 60034-3-Ig(Neprilysin/CD10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human tonsil using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsil using 60034-3-Ig(Neprilysin/CD10 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules Daudi, cellules HEK-293, cellules Ramos, tissu rénal de lapin, tissu rénal de porc, tissu rénal de souris
• Résultats positifs en IHC: tissu rénal humain, tissu amygdalien humain, tissu de cancer de l'estomac humain, tissu de carcinome à cellules rénales humain, tissu hépatique de souris, tissu hépatique humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:25000-1:100000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

60034-3-Ig cible Neprilysin/CD10 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, souris

• Réactivité: Humain, Lapin, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Neprilysin/CD10 Protéine recombinante Ag0427
• Nom complet: membrane metallo-endopeptidase
• Masse moléculaire calculée: 750 aa, 86 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC101658
• Symbole du gène: CD10
• Identification du gène (NCBI): 4311
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).

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