Anticorps Monoclonal anti-MME,CD10

• Phare
• Validé par KD/KO

MME,CD10 Monoclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, souris

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

4B9D9

N° de cat : 60034-3-Ig

Synonymes

Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, MME,CD10, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11

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Galerie de données de validation

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  WB analysis of HEK-293 using 60034-3-Ig

  WB result of MME,CD10 antibody (60034-3-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MME,CD10 transfected HEK-293 cells.

• 

  WB analysis of pig kidney using 60034-3-Ig

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 60034-3-Ig

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human renal cell carcinoma using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60034-3-Ig(MME,CD10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60034-3-Ig(MME,CD10 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60034-3-Ig (MME,CD10 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 60034-3-Ig(MME,CD10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 60034-3-Ig(MME,CD10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human tonsil using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsil using 60034-3-Ig(MME,CD10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human tonsil using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsil using 60034-3-Ig(MME,CD10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (MME,CD10 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 60034-3-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (MME,CD10 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Ramos using 60034-3-Ig

  1X10^6 Ramos cells were intracellularly stained with 0.2 ug Anti-Human MME,CD10 (60034-3-Ig, Clone:4B9D9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Raji using 60034-3-Ig

  1X10^6 Raji cells were intracellularly stained with 0.2 ug Anti-Human MME,CD10 (60034-3-Ig, Clone:4B9D9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu rénal de porc, cellules HEK-293, tissu rénal de souris
• Résultats positifs en IHC: tissu de carcinome à cellules rénales humain, tissu amygdalien humain, tissu hépatique de souris, tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules Ramos, cellules Raji

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

60034-3-Ig cible MME,CD10 dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MME,CD10 Protéine recombinante Ag0427
• Nom complet: membrane metallo-endopeptidase
• Masse moléculaire calculée: 750 aa, 86 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC101658
• Symbole du gène: MME
• Identification du gène (NCBI): 4311
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C