Anticorps Polyclonal de lapin anti-MMP1

MMP1 Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 26585-1-AP

Synonymes

CLG, CLGN, Fibroblast collagenase, Interstitial collagenase, Matrix metalloproteinase 1, MMP 1, MMP1

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Galerie de données de validation

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  WB analysis of A375 using 26585-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 26585-1-AP (MMP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 26585-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26585-1-AP (MMP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 26585-1-AP

  IP Result of anti-MMP1 (IP:26585-1-AP, 4ug; Detection:26585-1-AP 1:300) with Raji cells lysate 4000ug.

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  IHC staining of human cervical cancer using 26585-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 26585-1-AP (MMP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 26585-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 26585-1-AP (MMP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 26585-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26585-1-AP (MMP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 26585-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26585-1-AP (MMP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules NIH/3T3
• Résultats positifs en IP: cellules Raji
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

26585-1-AP cible MMP1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MMP1 Protéine recombinante Ag25073
• Nom complet: matrix metallopeptidase 1 (interstitial collagenase)
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: BC013875
• Symbole du gène: MMP1
• Identification du gène (NCBI): 4312
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MMP1, also named as CLG, belongs to the peptidase M10A family. MMP is the main enzyme that cleaves fibrillar collagen, namely types I, II, III, VII, and X.It is involved in cell migration and invasion, and is frequently up-regulated in cancer cells. It can be cleavage to two major forms (22 kDa and 27 kDa) by undergoing autolytic,and the minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.The sizes of pro-MMP-1 and active MMP-1 from human synovial fibroblasts have been reported as 52 to 56 kDa, depending on glycosylation, and 41 to 45 kDa, respectively.In addition ,a band of 62-kDa observed in the current study is pro-MMp-1(PMID:9418730).

Publications

Species	Application	Title
human,mouse	WB,IHC,IP	Breast Cancer Res Treat; Exosomal MMP-1 transfers metastasis potential in triple-negative breast cancer through PAR1-mediated EMT.; Authors - Yihui Zhu
human	WB	Front Genet; Exhausting circ_0136474 and Restoring miR-766-3p Attenuate Chondrocyte Oxidative Injury in IL-1β-Induced Osteoarthritis Progression Through Regulating DNMT3A.; Authors - Haiquan Zhu
		Mol Med Rep; Icariin inhibits MMP‑1, MMP‑3 and MMP‑13 expression through MAPK pathways in IL‑1β‑stimulated SW1353 chondrosarcoma cells.; Authors - Li Zeng