Anticorps Recombinant de lapin anti-MMP1

MMP1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230343G1

N° de cat : 83114-2-RR

Synonymes

22 kDa interstitial collagenase, 230343G1, 27 kDa interstitial collagenase, CLG, CLGN

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Galerie de données de validation

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  WB analysis of MDA-MB-231 using 83114-2-RR

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 83114-2-RR (MMP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 83114-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MMP1 antibody (83114-2-RR, Clone: 230343G1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 83114-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MMP1 Recombinant antibody (83114-2-RR, Clone:230343G1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83114-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83114-2-RR against Human MMP1 were performed. The affinity constant is 8.01 nM.

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Applications testées

• Résultats positifs en WB: cellules MDA-MB-231,
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83114-2-RR cible MMP1 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MMP1 Protéine recombinante Eg0527
• Nom complet: matrix metallopeptidase 1 (interstitial collagenase)
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 45-48 kDa
• Numéro d’acquisition GenBank: BC013875
• Symbole du gène: MMP1
• Identification du gène (NCBI): 4312
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MMP1, also named as CLG, belongs to the peptidase M10A family. MMP is the main enzyme that cleaves fibrillar collagen, namely types I, II, III, VII, and X.It is involved in cell migration and invasion, and is frequently up-regulated in cancer cells. It can be cleavage to two major forms (22 kDa and 27 kDa) by undergoing autolytic,and the minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.The sizes of pro-MMP-1 and active MMP-1 from human synovial fibroblasts have been reported as 52 to 56 kDa, depending on glycosylation, and 41 to 45 kDa, respectively.In addition ,a band of 62-kDa observed in the current study is pro-MMp-1(PMID:9418730).

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