Anticorps Polyclonal de lapin anti-MMP12

MMP12 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

27

Avis (3)

N° de cat : 22989-1-AP

Synonymes

HME, Macrophage elastase, Macrophage metalloelastase, Matrix metalloproteinase 12, ME, MME, MMP 12, MMP12

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Galerie de données de validation

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  WB analysis using 22989-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22989-1-AP (MMP12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 22989-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22989-1-AP (MMP12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22989-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22989-1-AP (MMP12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 22989-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22989-1-AP (MMP12 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 22989-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22989-1-AP (MMP12 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 22989-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22989-1-AP (MMP12 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 22989-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22989-1-AP (MMP12 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 22989-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 22989-1-AP (MMP12 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 22989-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 22989-1-AP (MMP12 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du sein humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below
• IHC: See 11 publications below
• IF: See 8 publications below

Informations sur le produit

22989-1-AP cible MMP12 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, Lapin, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MMP12 Protéine recombinante Ag19068
• Nom complet: matrix metallopeptidase 12 (macrophage elastase)
• Masse moléculaire calculée: 470 aa, 54 kDa
• Poids moléculaire observé: 54 kDa, 45 kDa
• Numéro d’acquisition GenBank: BC112301
• Symbole du gène: MMP12
• Identification du gène (NCBI): 4321
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MMP12(Matrix metalloproteinase-12) is a 54 kDa proenzyme that is processed into a 45 kDa and then a 22 kDa active form(15723202). MMP9 and MMP12 promote intimal thickening by independent cleavage of N-cadherin, which elevates vascular smooth muscle cell proliferation via beta-catenin signalling. Its overexpression in myeloid lineage cells plays a key role in modulating myelopoiesis, immune suppression, and lung tumorigenesis(PMID: 21378275).

Publications

Species	Application	Title
mouse	IF	Nat Cell Biol; Autophagy enables microglia to engage amyloid plaques and prevents microglial senescence; Authors - Insup Choi
human	WB,IHC	Nat Commun; Lung-derived HMGB1 is detrimental for vascular remodeling of metabolically imbalanced arterial macrophages.; Authors - Ludovic Boytard
mouse,human	WB,IF	Biomaterials; MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.; Authors - Mingming Song
mouse	WB,IF	J Crohns Colitis; Matrix Metalloproteinase MMP-12 promotes macrophage transmigration across intestinal epithelial tight junctions and increases severity of experimental colitis.; Authors - Meghali Nighot
human	WB	Redox Biol; Hydrogen sulfide attenuates cigarette smoke-induced airway remodeling by upregulating SIRT1 signaling pathway.; Authors - Ruijuan Guan
mouse	IHC	Respir Res; Role of PM2.5 in the development and progression of COPD and its mechanisms.; Authors - Junling Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hongxue (Verified Customer) (01-17-2023)

IHC staining in mouse liver, macrophage specific positive signaling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse liver

MMP12 Antibody Immunohistochemistry validation (1:500 dilution) in Mouse liver (Cat no:22989-1-AP)

FH

Yadavalli (Verified Customer) (07-20-2021)

I had used the MMP12 antibody and detected in the lung tissue which ad shown a significant data

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Lung

MMP12 Antibody Western Blot validation (1:1000 dilution) in Lung (Cat no:22989-1-AP)

FH

Hongxue (Verified Customer) (01-12-2021)

wb band very good

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Liver

MMP12 Antibody Western Blot validation (1:1000 dilution) in Liver (Cat no:22989-1-AP)