Anticorps Polyclonal de lapin anti-MMP14 / MT1-MMP

MMP14 / MT1-MMP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 29111-1-AP

Synonymes

Matrix metalloproteinase 14, MMP 14, MMP X1, MMP14, MT MMP 1, MT1 MMP, MT1MMP, MTMMP1

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Galerie de données de validation

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  WB analysis using 29111-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 29111-1-AP (MMP14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 29111-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29111-1-AP (MMP14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 29111-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29111-1-AP (MMP14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 29111-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using MMP14 antibody (29111-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549,
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

29111-1-AP cible MMP14 / MT1-MMP dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MMP14 / MT1-MMP Protéine recombinante Ag30755
• Nom complet: matrix metallopeptidase 14 (membrane-inserted)
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 66-70 kDa
• Numéro d’acquisition GenBank: BC064803
• Symbole du gène: MMP14
• Identification du gène (NCBI): 4323
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MMP14, also named as MT1-MMP, is a key matrix metalloproteinase (MMP) family member which plays a crucial role in tumor growth, invasion and metastasis. MT1-MMP is a cell membrane-bound proteinase, and it enhances degradation of collagen IV, a major component of the basement membrane, by forming a complex with tissue inhibitor of metalloproteinase-2 (TIMP-2) to activate pro-MMP-2. MT1-MMP can influence venous invasion, intrahepatic metastasis , and patient outcome in hepatocellular carcinoma (HCC). MT1-MMP was reported to be present in centromere and could lead to chromosome instability in MDCK cells, indicating that MT1-MMP may have more novel functions in the intracellular compartments. In western blotting, pro-MMP14 (65 kDa) and MMP14 (51 kDa) bands showed with the truncated MMP14 (45, 42, 35, 20 kDa) forms (PMID:12097451).

Publications

Species	Application	Title
human,mouse	WB	Bioact Mater; Biomimetic nanoparticles drive the mechanism understanding of shear-wave elasticity stiffness in triple negative breast cancers to predict clinical treatment; Authors - Dongdong Zheng
human,mouse	WB	Appl Biochem Biotechnol; Kunzea Ericoides (Kanuka) Leaf Extracts Show Moisturisation, Antioxidant, and UV Protection Effects in HaCaT Cells and Anti-melanogenesis Effects in B16F10 Cells; Authors - Xuefeng He
human	WB,IHC	Cancer Lett; Distinct Immunophenotypic Profiles and Neutrophil Heterogeneity in Colorectal Cancer; Authors - Minghua Bai
human	WB,IF	Front Oncol; Overexpression of MMP14 is associated with poor prognosis and immune cell infiltration in colon cancer; Authors - Na Li
mouse	WB	JCI Insight; LRP1 regulates asthmatic airway smooth muscle proliferation through FGF2/ERK signaling; Authors - Ya Deng

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