Anticorps Polyclonal de lapin anti-MMP21

MMP21 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 55289-1-AP

Synonymes

matrix metallopeptidase 21, Matrix metalloproteinase 21, MMP 21, MMP21

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Galerie de données de validation

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  WB analysis of mouse liver using 55289-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55289-1-AP (MMP21 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 55289-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 55289-1-AP (MMP21 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of L02 using 55289-1-AP

  Immunofluorescent analysis of L02 cells using 55289-1-AP (MMP21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules L02
• Résultats positifs en IF: cellules L02

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

55289-1-AP cible MMP21 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: matrix metallopeptidase 21
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: NM_147191
• Symbole du gène: MMP21
• Identification du gène (NCBI): 118856
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MMP21 belongs to the peptidase M10A family. It may have an important and specific function in tumor progression and embryogenesis. It cleaves alpha-1-antitrypsin. The proenzyme without the signal peptide has a calculated molecular mass of 62 kD; following activation by furin, the processed enzyme has a calculated molecular mass of 49 kD(PMID:12617721),and MMP21 protein was detected in fetal neuroectoderm, in placenta, and in leukocytes(OMIM).This antibody is specific to MMP21.

Publications

Species	Application	Title
mouse	IF	Nat Commun; Wnt activation disturbs cell competition and causes diffuse invasion of transformed cells through NF-κB-MMP21 pathway; Authors - Kazuki Nakai
human	WB	Hum Genomics; De novo disruptive heterozygous MMP21 variants are potential predisposing genetic risk factors in Chinese Han heterotaxy children; Authors - Xi-Ji Qin
human,mouse	WB,IHC	Cancer Sci; Matrix metalloproteinase-21 promotes metastasis via increasing the recruitment and M2 polarization of macrophages in HCC; Authors - Jiangfan Zhou ; KD Validated