Anticorps Recombinant de lapin anti-MMP3

MMP3 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

230535G8

N° de cat : 82984-4-RR

Synonymes

SL-1, MMP-3, MMP 3, Matrix metalloproteinase-3, EC:3.4.24.17

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Galerie de données de validation

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  WB analysis using 82984-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82984-4-RR (MMP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using 82984-4-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug MMP3 Recombinant antibody (82984-4-RR, Clone:230535G8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82984-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82984-4-RR against Human MMP3 were performed. The affinity constant is 0.348 nM.

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Applications testées

• Résultats positifs en WB: cellules MG U-87, cellules A2780
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

82984-4-RR cible MMP3 dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: matrix metallopeptidase 3 (stromelysin 1, progelatinase)
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC074815
• Symbole du gène: MMP3
• Identification du gène (NCBI): 4314
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Matrix metalloproteinases (MMPs) play a critically important role in extracellular matrix remodeling and have been implicated in a number of key normal and pathologic processes.These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 activate procollagenase via two pathways: slow direct activation and rapid activation in conjunction with tissue or plasma proteinases.

Publications

Species	Application	Title
mouse	WB,IHC	Bone Joint Res; Inhibition of PA28γ expression can alleviate osteoarthritis by inhibiting endoplasmic reticulum stress and promoting STAT3 phosphorylation; Authors - Haokun Mo

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