Anticorps Polyclonal de lapin anti-MMS19

• Phare
• Validé par KD/KO

MMS19 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(17)

N° de cat : 16015-1-AP

Synonymes

hMMS19, MET18, MET18 homolog, MMS19, MMS19 like protein, MMS19L

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Galerie de données de validation

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  WB analysis using 16015-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16015-1-AP (MMS19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16015-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16015-1-AP (MMS19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 16015-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16015-1-AP (MMS19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules PC-3, tissu cérébral humain
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 17 publications below
• CoIP: See 1 publications below

Informations sur le produit

16015-1-AP cible MMS19 dans les applications de WB, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MMS19 Protéine recombinante Ag8792
• Nom complet: MMS19 nucleotide excision repair homolog (S. cerevisiae)
• Masse moléculaire calculée: 1030 aa, 113 kDa
• Poids moléculaire observé: 103-113 kDa
• Numéro d’acquisition GenBank: BC006575
• Symbole du gène: MMS19
• Identification du gène (NCBI): 64210
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MMS19 plays a critical role in the biogenesis of multiple Fe-S proteins, which functions in genomic stability, RNA polymerase II function, and telomere length regulation. MMS19 has a alanine- and leucine-rich N terminus. It possible has a role in nucleotide excision repair(NER) and RNA polymerase II(POL II) transcription by interacting with ERCC2/XPB helicases, both subunits of NER-transcription factor TFIIH. Also MMS19 involves in the regulation of ER. It can function in chromosome segregation by acting as a part of the mitotic spindle-associated MMXD complex.

Publications

Species	Application	Title
human	WB	Cell; Hypoxia Rescues Frataxin Loss by Restoring Iron Sulfur Cluster Biogenesis.; Authors - Tslil Ast
human	WB	Mol Cell; Cytosolic Iron-Sulfur Assembly Is Evolutionarily Tuned by a Cancer-Amplified Ubiquitin Ligase.; Authors - Jenny L Weon ; KD Validated
human	WB	Cell Metab; Human CIA2A-FAM96A and CIA2B-FAM96B Integrate Iron Homeostasis and Maturation of Different Subsets of Cytosolic-Nuclear Iron-Sulfur Proteins.; Authors - Stehling Oliver O ; KD Validated
mouse	WB	Nat Commun; Structural basis for substrate recognition and chemical inhibition of oncogenic MAGE ubiquitin ligases.; Authors - Seung Wook Yang
human	WB	Cell Rep; The CIA Targeting Complex Is Highly Regulated and Provides Two Distinct Binding Sites for Client Iron-Sulfur Proteins.; Authors - Diana C Odermatt ; KD Validated
mouse	WB	Mol Metab; Cdkal1, a type 2 diabetes susceptibility gene, regulates mitochondrial function in adipose tissue.; Authors - Colin J Palmer

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