Anticorps Polyclonal de lapin anti-MOF

• Phare
• Validé par KD/KO

MOF Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF/ICC, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 13842-1-AP

Synonymes

MYST1, Lysine acetyltransferase 8, KAT8, hMOF, Histone acetyltransferase KAT8

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Galerie de données de validation

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  WB analysis of HeLa using 13842-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13842-1-AP (MOF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 13842-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 13842-1-AP (MOF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13842-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13842-1-AP (MOF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13842-1-AP

  IP result of anti-MOF (IP:13842-1-AP, 4ug; Detection:13842-1-AP 1:600) with HeLa cells lysate 1200ug.

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  IF Staining of HCT 116 using 13842-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using MOF antibody (13842-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, tissu hépatique de rat
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules HCT 116,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

13842-1-AP cible MOF dans les applications de WB, IF/ICC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris, Ver à soie
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MOF Protéine recombinante Ag4802
• Nom complet: MYST histone acetyltransferase 1
• Masse moléculaire calculée: 467 aa, 53 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC037773
• Symbole du gène: KAT8/MOF
• Identification du gène (NCBI): 84148
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MOF, also known as MYST1, KAT8, is a member of the MYST acetyltransferase family, which is homologous to Drosophila males absent on the first (MOF) protein. Deletion of MYST1 in cells leads to genomic instability, spontaneous chromosomal aberration, cell cycle defects, reduced transcription of certain genes and defective DNA damage repair (PMID: 32711345). MYST1 dually localizes to the nucleus and mitochondria of human cancer cells (PMID: 37813994). Western blot analysis detected MOF at an apparent molecular mass of 52 kDa.

Publications

Species	Application	Title
human	WB	Nature; A druggable copper-signalling pathway that drives inflammation; Authors - Stéphanie Solier
Mouse	WB	Nat Commun; Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.; Authors - I-Ling Lu
human	WB,IP	Autophagy; KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.; Authors - Guokai Yan
human	WB,ChIP	Redox Biol; Serum response factor (SRF) promotes ROS generation and hepatic stellate cell activation by epigenetically stimulating NCF1/2 transcription.; Authors - Ming Kong ; KD Validated
human	WB	Mol Cell Proteomics; Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm).; Authors - Heguo Yu
human	WB	Mol Cell Proteomics; Regulation of acetylation restores proteolytic function of diseased myocardium in mouse and human.; Authors - Ding Wang

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