Anticorps Polyclonal de lapin anti-MOSC2

MOSC2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 24782-1-AP

Synonymes

MOSC domain-containing protein 2, Molybdenum cofactor sulfurase C-terminal domain-containing protein 2, Moco sulfurase C-terminal domain-containing protein 2, Mitochondrial amidoxime reducing component 2, mARC2

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Galerie de données de validation

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  WB analysis using 24782-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24782-1-AP (MOSC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24782-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24782-1-AP (MOSC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 24782-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24782-1-AP (MOSC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 24782-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24782-1-AP (MOSC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 24782-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24782-1-AP (MOSC2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 24782-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24782-1-AP (MOSC2 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de rat
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

24782-1-AP cible MOSC2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MOSC2 Protéine recombinante Ag20694
• Nom complet: MOCO sulphurase C-terminal domain containing 2
• Masse moléculaire calculée: 335 aa, 38 kDa
• Poids moléculaire observé: 35-38 kDa
• Numéro d’acquisition GenBank: BC011973
• Symbole du gène: MOSC2
• Identification du gène (NCBI): 54996
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MOSC domain-containing protein 2 (also known as MOSC2), also known as MARC2, is a component of prodrug-converting system, reduces a multitude of N-hydroxylated prodrugs particularly amidoximes, leading to increased drug bioavailability. Also, MOSC2 may be involved in mitochondrial N(omega)-hydroxy-L-arginine (NOHA) reduction, regulating endogenou1s nitric oxide levels and biosynthesis. The reductase activity is regulated under adipogenic conditions, and down-regulation of the terminal component MOSC2 resulted in decreased lipid synthesis, suggesting a possible physiological role of this enzyme system and its component MOSC2 in lipogenesis(PMID: 22203676).

Publications

Species	Application	Title
human	WB	Cancers (Basel); RNA Sequencing of Collecting Duct Renal Cell Carcinoma Suggests an Interaction between miRNA and Target Genes and a Predominance of Deregulated Solute Carrier Genes.; Authors - Sven Wach
		PLoS Genet; Divergent role of Mitochondrial Amidoxime Reducing Component 1 (MARC1) in human and mouse; Authors - Eriks Smagris

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